Cloned (Comment) | Organism |
---|---|
gene asd, DNA and amino acid sequence determination and analysis, sequence comparisons | Pseudomonas sp. |
Protein Variants | Comment | Organism |
---|---|---|
D360P | site-directed mutagenesis, the mutant shows similar 4-decarboxylation activity but reduced aminotransferase compared to the wild-type enzyme | Pseudomonas sp. |
F204W | site-directed mutagenesis, the mutant shows slightly reduced 4-decarboxylation activity but highly increased aminotransferase compared to the wild-type enzyme | Pseudomonas sp. |
H123Y | site-directed mutagenesis, the mutant shows reduced 4-decarboxylation activity and slightly increased aminotransferase compared to the wild-type enzyme | Pseudomonas sp. |
H337R | site-directed mutagenesis, the mutant shows reduced 4-decarboxylation activity but similar aminotransferase compared to the wild-type enzyme | Pseudomonas sp. |
K347R | site-directed mutagenesis, the mutant shows similar 4-decarboxylation activity but reduced aminotransferase compared to the wild-type enzyme | Pseudomonas sp. |
M178L | site-directed mutagenesis, the mutant shows reduced 4-decarboxylation activity but unaltered aminotransferase compared to the wild-type enzyme | Pseudomonas sp. |
V475L | site-directed mutagenesis, the mutant shows slightly reduced 4-decarboxylation activity but unaltered aminotransferase compared to the wild-type enzyme | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | Pseudomonas sp. | - |
L-alanine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | Q53IZ1 | strain ATCC 19121 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
4-decarboxylation activity and aminotransferase activity of mutant enzymes, overview | Pseudomonas sp. |
0.06 | - |
recombinant wild-type enzyme, aminotransferase activity | Pseudomonas sp. |
128.8 | - |
recombinant wild-type enzyme, 4-decarboxylation activity | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | - |
Pseudomonas sp. | L-alanine + CO2 | - |
? | |
additional information | Asd is a bifunctional enzyme performing decarboxylation and aminotransferase activities on L-aspartate | Pseudomonas sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Asd | - |
Pseudomonas sp. |
L-aspartate 4-decarboxylase | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Pseudomonas sp. |