Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Methanosarcina barkeri |
expressed in Escherichia coli BL21(DE3) cells | Desulfovibrio desulfuricans |
expressed in Escherichia coli BL21(DE3) cells | Desulfovibrio vulgaris |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.2 M ammonium acetate, 0.1 M sodium acetate pH 4.5, 30% (w/v) PEG 4000, 0.01 M BaCl2 | Desulfovibrio desulfuricans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
siroheme | - |
Desulfovibrio desulfuricans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01174 | - |
siroheme | pH and temperature not specified in the publication | Desulfovibrio desulfuricans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
calculated from amino acid sequence | Desulfovibrio desulfuricans |
40000 | - |
gel filtration | Methanosarcina barkeri |
40000 | - |
gel filtration | Desulfovibrio desulfuricans |
40000 | - |
gel filtration | Desulfovibrio vulgaris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
siroheme | Methanosarcina barkeri | - |
12,18-didecarboxysiroheme + CO2 | - |
? | |
siroheme | Desulfovibrio desulfuricans | - |
12,18-didecarboxysiroheme + CO2 | - |
? | |
siroheme | Desulfovibrio vulgaris | - |
12,18-didecarboxysiroheme + CO2 | - |
? | |
siroheme | Desulfovibrio desulfuricans ATCC27774 | - |
12,18-didecarboxysiroheme + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfovibrio desulfuricans | - |
- |
- |
Desulfovibrio desulfuricans ATCC27774 | - |
- |
- |
Desulfovibrio vulgaris | - |
- |
- |
Methanosarcina barkeri | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose column chromatography and Superdex 200 gel filtration | Methanosarcina barkeri |
Ni-Sepharose column chromatography and Superdex 200 gel filtration | Desulfovibrio desulfuricans |
Ni-Sepharose column chromatography and Superdex 200 gel filtration | Desulfovibrio vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
siroheme | - |
Methanosarcina barkeri | 12,18-didecarboxysiroheme + CO2 | - |
? | |
siroheme | - |
Desulfovibrio desulfuricans | 12,18-didecarboxysiroheme + CO2 | - |
? | |
siroheme | - |
Desulfovibrio vulgaris | 12,18-didecarboxysiroheme + CO2 | - |
? | |
siroheme | - |
Desulfovibrio desulfuricans ATCC27774 | 12,18-didecarboxysiroheme + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | - |
Methanosarcina barkeri |
heterodimer | - |
Desulfovibrio desulfuricans |
heterodimer | - |
Desulfovibrio vulgaris |
Synonyms | Comment | Organism |
---|---|---|
AhbA | subunit | Methanosarcina barkeri |
AhbA | subunit | Desulfovibrio desulfuricans |
AhbA | subunit | Desulfovibrio vulgaris |
AhbB | subunit | Methanosarcina barkeri |
AhbB | subunit | Desulfovibrio desulfuricans |
AhbB | subunit | Desulfovibrio vulgaris |
sirohaem decarboxylase | - |
Methanosarcina barkeri |
sirohaem decarboxylase | - |
Desulfovibrio desulfuricans |
sirohaem decarboxylase | - |
Desulfovibrio vulgaris |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00177 | - |
siroheme | pH and temperature not specified in the publication | Desulfovibrio desulfuricans |