Cloned (Comment) | Organism |
---|---|
gene panD, expression of enzyme mutant N72A in Escherichia coli strain C41 (DE3) | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
purified recombinant mutant N72A, hanging drop vapour diffusion method, mixing of 0.001 ml of 19 mg /ml protein in 50 mM Tris-HCl, pH 8.0, with 0.001 ml of reservoir solution containing 1.6 M ammonium sulfate, pH 4.0, and equilibration against 0.5 ml of reservoir solution, 19°C, 3 days, cryoprotection by 1.8 M ammonium sulfate, 0.1 M citric acid, 30% glycerol pH 4.0 using 5% increments in glycerol concentration to prevent crystal dissolution, X-ray diffraction structure determination and analysis at 1.7 A resolution | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
N72A | site-directed mutagenesis, in the Asn72Ala mutant the C-terminal region residues are ordered, in contrast to the wild-type enzyme, owing to an interaction with the active site of the neighbouring symmetry-related multimer | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | Escherichia coli | - |
beta-alanine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A790 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme mutant N72A from Escherichia coli strain C41 (DE3) to homogeneity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | - |
Escherichia coli | beta-alanine + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADC | - |
Escherichia coli |
Aspartate alpha-decarboxylase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyruvoyl cofactor | the pyruvoyl group is required for catalysis | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of a small class of pyruvoyl-dependent decarboxylases, in which the enzyme-bound pyruvoyl cofactor is generated via the autocatalytic rearrangement of a serine residue via an ester intermediate | Escherichia coli |