Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
A287G | the mutant shows reduced activity compared to the wild type enzyme | Saccharomyces cerevisiae |
C221A/C222A | the mutant shows reduced activity compared to the wild type enzyme | Saccharomyces cerevisiae |
H225F | the mutant shows reduced activity compared to the wild type enzyme | Saccharomyces cerevisiae |
H310F | the mutant shows reduced activity compared to the wild type enzyme | Saccharomyces cerevisiae |
H92F | the mutant shows wild type activity | Saccharomyces cerevisiae |
S311A | the mutant shows reduced activity compared to the wild type enzyme | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
pyruvate | mutant enzyme A287G, at pH 6.0 and 30°C | Saccharomyces cerevisiae | |
0.1 | - |
pyruvate | mutant enzyme S311A, at pH 6.0 and 30°C | Saccharomyces cerevisiae | |
0.3 | - |
pyruvate | mutant enzyme H92F, at pH 6.0 and 30°C | Saccharomyces cerevisiae | |
0.4 | - |
pyruvate | mutant enzyme C221A/C222A, at pH 6.5 and 10°C | Saccharomyces cerevisiae | |
0.5 | - |
pyruvate | wild type enzyme, at pH 6.0 and 30°C | Saccharomyces cerevisiae | |
16 | - |
pyruvate | mutant enzyme H310F, at pH 6.0 and 30°C | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | Saccharomyces cerevisiae | - |
acetaldehyde + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography, Fractogel EMD TMAE 650S gel filtration, and Superdex 200 gel filtration | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | - |
Saccharomyces cerevisiae | acetaldehyde + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-oxo acid carboxylyase | - |
Saccharomyces cerevisiae |
PDC | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | dependent on | Saccharomyces cerevisiae |