Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Komagataella pastoris |
expressed in Escherichia coli BL21(DE3)pLysS cells | Saccharomyces cerevisiae |
subcloning in Escherichia coli strain DH5alpha, expression in Escherichia coli strain BL21(DE3)plysS | Komagataella pastoris |
subcloning in Escherichia coli strain DH5alpha, expression of C-terminally His-tagged isozymes in Escherichia coli strain BL21(DE3)plysS | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | growth profile and ethanol production in isozyme knockout strains, overview | Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
the highest enzyme activity is in glucose, followed by glycerol, while there is negligible activity on ethanol and methanol as growth medium | Komagataella pastoris |
the highest enzyme activity is in glucose, followed by glycerol, while there is negligible activity on ethanol and methanol as growth medium | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Komagataella pastoris | |
Mg2+ | required | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
x * 66000, SDS-PAGE | Komagataella pastoris |
66000 | - |
x * 66000, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | apart from the decarboxylation reaction, pyruvate decarboxylases are also known for their carboligation capabilities. During this reaction, the active aldehyde in the active site is condensed with a second aldehyde as a cosubstrate to form hydroxy ketones, when the co-substrate is acetaldehyde, (R)-acetoin is formed | ? | - |
? | |
additional information | Saccharomyces cerevisiae BY4741 | apart from the decarboxylation reaction, pyruvate decarboxylases are also known for their carboligation capabilities. During this reaction, the active aldehyde in the active site is condensed with a second aldehyde as a cosubstrate to form hydroxy ketones, when the co-substrate is acetaldehyde, (R)-acetoin is formed | ? | - |
? | |
pyruvate | Saccharomyces cerevisiae | - |
acetaldehyde + CO2 | - |
? | |
pyruvate | Komagataella pastoris | - |
acetaldehyde + CO2 | - |
? | |
pyruvate | Saccharomyces cerevisiae BY4741 | - |
acetaldehyde + CO2 | - |
? | |
pyruvate | Komagataella pastoris GS115 | - |
acetaldehyde + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Komagataella pastoris | - |
- |
- |
Komagataella pastoris | C4R3T2 | i.e. Komagataella pastoris, has a single isozyme | - |
Komagataella pastoris GS115 | C4R3T2 | i.e. Komagataella pastoris, has a single isozyme | - |
Saccharomyces cerevisiae | P06169 | - |
- |
Saccharomyces cerevisiae | P06169 | isozyme PDC1; pyruvate decarboxylase isozyme 1 | - |
Saccharomyces cerevisiae | P16467 | - |
- |
Saccharomyces cerevisiae | P16467 | isozyme PDC5; pyruvate decarboxylase isozyme 2, PDC5 | - |
Saccharomyces cerevisiae | P26263 | - |
- |
Saccharomyces cerevisiae | P26263 | isozyme PDC6; pyruvate decarboxylase isozyme 3, PDC6 | - |
Saccharomyces cerevisiae BY4741 | P06169 | isozyme PDC1; pyruvate decarboxylase isozyme 1 | - |
Saccharomyces cerevisiae BY4741 | P16467 | isozyme PDC5; pyruvate decarboxylase isozyme 2, PDC5 | - |
Saccharomyces cerevisiae BY4741 | P26263 | isozyme PDC6; pyruvate decarboxylase isozyme 3, PDC6 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | growth profile and ethanol production in isozyme knockout strains, overview | Saccharomyces cerevisiae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
comparison of decarboxylation activities of the PDC isozymes, PDC5 shows the highest activity, overview | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | apart from the decarboxylation reaction, pyruvate decarboxylases are also known for their carboligation capabilities. During this reaction, the active aldehyde in the active site is condensed with a second aldehyde as a cosubstrate to form hydroxy ketones, when the co-substrate is acetaldehyde, (R)-acetoin is formed | Saccharomyces cerevisiae | ? | - |
? | |
additional information | apart from the decarboxylation reaction, pyruvate decarboxylases are also known for their carboligation capabilities. During this reaction, the active aldehyde in the active site is condensed with a second aldehyde as a cosubstrate to form hydroxy ketones, when the co-substrate is acetaldehyde, (R)-acetoin is formed | Saccharomyces cerevisiae BY4741 | ? | - |
? | |
pyruvate | - |
Komagataella pastoris | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Saccharomyces cerevisiae | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Saccharomyces cerevisiae BY4741 | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Komagataella pastoris GS115 | acetaldehyde + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 66000, SDS-PAGE | Komagataella pastoris |
? | x * 66000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
PDC | - |
Komagataella pastoris |
PDC | - |
Saccharomyces cerevisiae |
PDC1 | - |
Komagataella pastoris |
PDC1 | isoform | Saccharomyces cerevisiae |
Pdc5 | isoform | Saccharomyces cerevisiae |
Pdc6 | isoform | Saccharomyces cerevisiae |
scpdc1 | - |
Saccharomyces cerevisiae |
ScPDC5 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.6 | - |
pyruvate | at pH 7.0 and 25°C | Komagataella pastoris | |
65 | - |
pyruvate | isoform PDC6, at pH 7.0 and 25°C | Saccharomyces cerevisiae | |
145 | - |
pyruvate | isoform PDC1, at pH 7.0 and 25°C | Saccharomyces cerevisiae | |
207 | - |
pyruvate | isoform PDC5, at pH 7.0 and 25°C | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Komagataella pastoris | |
thiamine diphosphate | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
physiological function | roles of the three isozymes at different phases of Saccharomyces cerevisiae fermentation and with relevance for ethanol and carboligation reactions, overview | Saccharomyces cerevisiae |