Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.1 extracted from

  • Rivoal, J.; Ricard, B.; Pradet, A.
    Purification and partial characterization of pyruvate decarboxylase from Oryza sativa L. (1990), Eur. J. Biochem., 194, 791-797.
    View publication on PubMed
Search for more literature for 4.1.1.1

Inhibitors

Inhibitors Comment Organism Structure
(E)-4-(4-Chlorophenyl)-2-oxo-3-butenoic acid reversible Oryza sativa
glyoxalate
-
 Oryza sativa
phosphate
-
 Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
220000 240000 gel filtration Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oryza sativa

Renatured (Commentary)

Renatured (Comment) Organism
renaturation of apoenzyme with MgCl2 is achieved by incubation of dialyzed enzyme with 1 mM thiamine diphosphate and increasing concentrations of MgCl2 Oryza sativa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate
-
 Oryza sativa acetaldehyde + CO2
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 62000-64000, SDS-PAGE Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
-
 Oryza sativa

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7 pH 5.5: about 60% of maximal activity, pH 7.0: about 45% of maximal activity Oryza sativa

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
 above pH 8 the enzyme dissociates into two dimeric subunits Oryza sativa