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Literature summary for 3.8.1.5 extracted from
- A Novel mini protein design of haloalkane dehalogenase (2019), Mol. Biotechnol., 61, 477-488 .
Application
| Application | Comment | Organism |
|---|---|---|
| industry | a stable mini protein is successfully designed and may be used as bioreceptors in the haloalkane sensor that is suitable for industrial application | Xanthobacter autotrophicus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 (DE3) | Xanthobacter autotrophicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Xanthobacter autotrophicus | P22643 | - |
- |
| Xanthobacter autotrophicus GJ10 | P22643 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| five mini proteins are designed via computer modeling and analysis that mimick the main function of haloalkane dehalogenase. A mini protein with 86 amino acids is chosen for His-tag purification | Xanthobacter autotrophicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,3-dibromopropane + H2O | - |
Xanthobacter autotrophicus | 3-bromopropanol + bromide | - |
? |  |
| 1,3-dibromopropane + H2O | - |
Xanthobacter autotrophicus GJ10 | 3-bromopropanol + bromide | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 43.97 | - |
native enzyme | Xanthobacter autotrophicus |
| 83.73 | - |
mini protein, designed via computer modeling and analysis that mimicks the main function of haloalkane dehalogenase | Xanthobacter autotrophicus |
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Release 2023.1 (January 2023)
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