Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
x * 29000, SDS-PAGE of mercaptoethanol denatured enzyme | Pseudomonas cruciviae |
160000 | - |
gel filtration | Pseudomonas cruciviae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas cruciviae | enzyme is involved in the degradation of biphenyl | ? | - |
? | |
additional information | Pseudomonas cruciviae S93 B1 | enzyme is involved in the degradation of biphenyl | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas cruciviae | - |
S93 B1 | - |
Pseudomonas cruciviae S93 B1 | - |
S93 B1 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas cruciviae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
51.39 | - |
- |
Pseudomonas cruciviae |
Storage Stability | Organism |
---|---|
4°C, pH 7.4, 10% loss of activity after 1 month | Pseudomonas cruciviae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-isopropylcatechol + H2O | - |
Pseudomonas cruciviae | ? | - |
? | |
3-isopropylcatechol + H2O | - |
Pseudomonas cruciviae S93 B1 | ? | - |
? | |
3-methylcatechol + H2O | - |
Pseudomonas cruciviae | ? | - |
? | |
3-methylcatechol + H2O | - |
Pseudomonas cruciviae S93 B1 | ? | - |
? | |
biphenyl-2,3-diol + H2O | - |
Pseudomonas cruciviae | ? | - |
? | |
biphenyl-2,3-diol + H2O | - |
Pseudomonas cruciviae S93 B1 | ? | - |
? | |
catechol + H2O | - |
Pseudomonas cruciviae | ? | - |
? | |
catechol + H2O | - |
Pseudomonas cruciviae S93 B1 | ? | - |
? | |
additional information | enzyme is involved in the degradation of biphenyl | Pseudomonas cruciviae | ? | - |
? | |
additional information | enzyme is involved in the degradation of biphenyl | Pseudomonas cruciviae S93 B1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, SDS-PAGE of mercaptoethanol denatured enzyme | Pseudomonas cruciviae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
1 h, stable | Pseudomonas cruciviae |
60 | - |
pH 7.4, 5 min, 75% loss of activity | Pseudomonas cruciviae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Pseudomonas cruciviae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.7 | pH 6.5: about 65% of maximal activity, pH 8.7: about 25% of maximal activity | Pseudomonas cruciviae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9 | 11 | 25°C, 6 h, stable | Pseudomonas cruciviae |