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Literature summary for 3.7.1.13 extracted from

  • Riddle, R.R.; Gibbs, P.R.; Willson, R.C.; Benedik, M.J.
    Purification and properties of 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoic acid hydrolase involved in microbial degradation of carbazole (2003), Protein Expr. Purif., 28, 182-189.
    View publication on PubMed
Search for more literature for 3.7.1.13

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas sp.

Protein Variants

Protein Variants Comment Organism
S114A mutation in active-site serine. Approximately 40fold reduction in activity for the His-tagged mutant hydrolase relative to similar lysates of His-tagged native CarC Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0046
-
 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid pH 7.5, 25°C Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32231
-
 x * 32231, calculated, x * 33000, SDS-PAGE Pseudomonas sp.
33000
-
 x * 32231, calculated, x * 33000, SDS-PAGE Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. LD2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2600
-
 pH 7.5, 25°C Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid + H2O
-
 Pseudomonas sp. benzoic acid + 2-hydroxypenta-2,4-dienoic acid
-
 ?
2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid + H2O
-
 Pseudomonas sp. LD2 benzoic acid + 2-hydroxypenta-2,4-dienoic acid
-
 ?

Subunits

Subunits Comment Organism
? x * 32231, calculated, x * 33000, SDS-PAGE Pseudomonas sp.

Synonyms

Synonyms Comment Organism
HOPDA hydrolase
-
 Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
58
-
-
 Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1300
-
 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid pH 7.5, 25°C Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.5
-
 no activity Pseudomonas sp.
9
-
 about 50% of maximal activity Pseudomonas sp.
10.5
-
 no activity Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor requirement Pseudomonas sp.