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Literature summary for 3.6.5.5 extracted from

  • Lomash, R.M.; Petralia, R.S.; Holtzclaw, L.A.; Tsuda, M.C.; Wang, Y.X.; Badger, J.D.; Cameron, H.A.; Youle, R.J.; Roche, K.W.
    Neurolastin, a dynamin family GTPase, translocates to mitochondria upon neuronal stress and alters mitochondrial morphology in vivo (2019), J. Biol. Chem., 294, 11498-11512 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.5.5

Cloned(Commentary)

Cloned (Comment) Organism
gene Rnf112, recombinant expression of C-terminally HA-tagged enzyme in transgenic C83S/C103S mutant mice and in HeLa cells, and of GFP-tagged enzyme from pEGFP-N1 vector in transgenic mice Mus musculus

Protein Variants

Protein Variants Comment Organism
C83S/C103S site-directed mutagenesis Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial intermembrane space neurolastin is developmentally regulated and present on mitochondria. Neurolastin also localizes to mitochondria in transgenic HeLa cells, cultured neurons, and brain tissue. The mitochondrial localization of neurolastin depends upon an N-terminal mitochondrial targeting sequence. Neurolastin is imported into the mitochondrial intermembrane space. Although neurolastin is only partially mitochondrially localized at steady state, it displays increased translocation to mitochondria in response to neuronal stress and mitochondrial fragmentation Mus musculus 5758
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additional information determination of colocalization of recominant GFP-tagged enzyme with mitochondria in neurons and brains of transgenic mice. Immunohistochemic analysis Mus musculus
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Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Mus musculus
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 GDP + phosphate
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 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q8K1M6
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-

Posttranslational Modification

Posttranslational Modification Comment Organism
ubiquitination ubiquitin-mediated regulation of dynamin GTPases serves as a quality control mechanism for maintaining mitochondrial dynamics Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
brain neuolastin is specifically expressed in the brain Mus musculus
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neuron primary hippocampal neurons, isolated from P0-P1 pups of transgenic animals expressing HA-tagged enzyme Mus musculus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Mus musculus GDP + phosphate
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 ?
additional information neurolastin exhibits both GTPase and E3 ligase activities Mus musculus ?
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Synonyms

Synonyms Comment Organism
dynamin GTPase
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 Mus musculus

General Information

General Information Comment Organism
evolution neurolastin is a dynamin family GTPase. It also contains a RING domain and exhibits both GTPase and E3 ligase activities Mus musculus
malfunction neurolastin knockout animals have fewer dendritic spines and exhibit a reduction in functional synapses Mus musculus
physiological function neurolastin, a dynamin family GTPase, translocates to mitochondria upon neuronal stress and alters mitochondrial morphology in vivo. It is specifically expressed in the brain and is important for synaptic transmission Mus musculus