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Literature summary for 3.6.5.5 extracted from
- Distinct splice variants of dynamin-related protein 1 differentially utilize mitochondrial fission factor as an effector of cooperative GTPase activity (2016), J. Biol. Chem., 291, 493-507 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cardiolipin | stimulates Drp1, synergistically with mitochondrial fission factor | Homo sapiens |  |
| cardiolipin | stimulates Drp1, synergistically with mitochondrial fission factor | Rattus norvegicus | |
| Mff-proteoliposome | lipid-stimulated GTPase activity | Homo sapiens | |
| Mff-proteoliposome | lipid-stimulated GTPase activity | Rattus norvegicus | |
| mitochondrial fission factor | Mff, stimulates Drp1 independently of, but synergistically with cardiolipin | Homo sapiens | |
| mitochondrial fission factor | Mff, stimulates Drp1 independently of, but synergistically with cardiolipin | Rattus norvegicus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene DNMTL1, recombinant expression of the splice variants of Drp1 in Escherichia coli strain BL21 Star (DE3) | Homo sapiens |
| gene DNMTL1, recombinant expression of the splice variants of Drp1 in Escherichia coli strain BL21 Star (DE3) | Rattus norvegicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | Drp1-A-only (712 aa, 699 aax0413-aa A-insert) and Drp1-B-only (742 aa, 755 aa, 13-aa A-insert) are introduced in the Drp1-long isozyme. A- and B-insert sequences both suppress Drp1 GTPase activity, with the B-insert exerting the most dominant inhibitory effect on these interactions | Rattus norvegicus |
| additional information | Drp1-A-only (712 aa, 699 aax0413-aa A-insert) and Drp1-B-only (742 aa, 755 aa, 13-aa A-insert) are introduced in the Drp1-short isozyme. A- and B-insert sequences both suppress Drp1 GTPase activity, with the B-insert exerting the most dominant inhibitory effect on these interactions | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Homo sapiens | |
| additional information | - |
additional information | Michaelis-Menten kinetics | Rattus norvegicus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens | |
| Mg2+ | required | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | Homo sapiens | - |
GDP + phosphate | - |
? | |
| GTP + H2O | Rattus norvegicus | - |
GDP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O00429 | - |
- |
| Rattus norvegicus | O35303 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | - |
Homo sapiens | GDP + phosphate | - |
? | |
| GTP + H2O | - |
Rattus norvegicus | GDP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | Drp1 isoforms exhibit differential oligomerization propensities in solution and distinct helical geometry on cardiolipin-containing membranes, analysis of Drp1-long, Drp1-A-only, and Drp1-B-only by gel filtration | Rattus norvegicus |
| More | Drp1 isoforms exhibit differential oligomerization propensities in solution and distinct helical geometry on cardiolipin-containing membranes, analysis of Drp1-short, Drp1-A-only, and Drp1-B-only by gel filtration | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DNM1L | - |
Homo sapiens |
| DNM1L | - |
Rattus norvegicus |
| Drp1-long | - |
Rattus norvegicus |
| Drp1-short | isozyme 3 | Homo sapiens |
| dynamin-1-like protein | - |
Rattus norvegicus |
| dynamin-related protein 1 | - |
Homo sapiens |
| dynamin-related protein 1 | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
| 7.5 | - |
assay at | Rattus norvegicus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | Drp1 isoforms exhibit differential GTPase activities, comparison of splice variants, overview. Human isozyme Drp1-short exhibits greater cooperative GTPase activities relative to rat Drp1-long | Homo sapiens |
| additional information | Drp1 isoforms exhibit differential GTPase activities, comparison of splice variants, overview. Human isozyme Drp1-short exhibits greater cooperative GTPase activities relative to rat Drp1-long | Rattus norvegicus |
| physiological function | distinct splice variants of dynamin-related protein 1 differentially utilize mitochondrial fission factor as an effector of cooperative GTPase activity | Homo sapiens |
| physiological function | distinct splice variants of dynamin-related protein 1 differentially utilize mitochondrial fission factor as an effector of cooperative GTPase activity | Rattus norvegicus |
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