Cloned (Comment) | Organism |
---|---|
full-length, partial cDNA fragments and point mutations of mouse NSF are amplified by PCR and subcloned into different expression vectors including pGEX-6P-2, pETH-32, pcDNA3-His6, and pcDNA3-3HA | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
S569A | mutation of serine 569 to alanine abolishs the phosphorylation of D2 domain by active Pctaire1 | Mus musculus |
S569A | mutation of serine 569 to alanine abolishs the phosphorylation of D2 domain by active Pctaire1 | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | phosphorylation by Pctaire1 attenuates the hexamerization of NSF | Mus musculus | |
additional information | phosphorylation by Pctaire1 attenuates the hexamerization of NSF | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
cytosol | - |
Rattus norvegicus | 5829 | - |
membrane | NSF requires a peripheral membrane protein to bind membranes | Mus musculus | 16020 | - |
membrane | NSF requires a peripheral membrane protein to bind membranes | Rattus norvegicus | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Mus musculus | - |
ADP + phosphate | - |
ir | |
ATP + H2O | Rattus norvegicus | - |
ADP + phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylated by Pctaire1 on serine 569 | Mus musculus |
phosphoprotein | phosphorylated by Pctaire1 on serine 569 | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
protein extracts from cells and brain tissues are prepared | Rattus norvegicus |
recombinant NSF is purified by nickel-nitrilotriacetic acid-agarose affinity chromatography | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
nervous system | - |
Rattus norvegicus | - |
PC-12 cell | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Mus musculus | ADP + phosphate | - |
ir | |
ATP + H2O | - |
Rattus norvegicus | ADP + phosphate | - |
ir |
Subunits | Comment | Organism |
---|---|---|
homohexamer | - |
Mus musculus |
homohexamer | - |
Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
N-ethylmaleimide-sensitive fusion protein | - |
Mus musculus |
N-ethylmaleimide-sensitive fusion protein | - |
Rattus norvegicus |
NSF | - |
Mus musculus |
NSF | - |
Rattus norvegicus |