Cloned (Comment) | Organism |
---|---|
expression of ClpX lacking the N-terminal Cys cluster domain, i.e. residues 71-446 in Escherichia coli | Helicobacter pylori |
Crystallization (Comment) | Organism |
---|---|
recombinant ClpX lacking the N-terminal Cys cluster domain, i.e. residues 71-446, hanging drop vapor diffusion at 22°C, reservoir solution contains 100 mM imidazole, pH 8.0, 1 M (NH4)2HPO4 and 200 mM NaCl, to obtain crystals of ClpX complexed with nucleotides, 5 mM ADP or ATPgammaS and MgCl2 are added, crystals diffract to 2.6 A | Helicobacter pylori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Helicobacter pylori | - |
ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant ClpX lacking the N-terminal Cys cluster domain, i.e. residues 71-446, Q-Sepharose, Superdex-200 | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Helicobacter pylori | ADP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ClpX | - |
Helicobacter pylori |