Literature summary for 3.6.1.9 extracted from

Hluska, T.; Sebela, M.; Lenobel, R.; Frebort, I.; Galuszka, P.
Purification of maize nucleotide pyrophosphatase/phosphodiesterase casts doubt on the existence of zeatin cis-trans isomerase in plants (2017), Front. Plant Sci., 8, 1473 .

Search for more literature for 3.6.1.9

Cloned(Commentary)

Cloned (Comment)	Organism
gene ZmNPP1, DNA and amino acid sequence determination and analysis, the gene contains a single exon, phylogenetic analysis and tree, recombinant overexpresison in Arabidopsis thaliana plants, and recombinant expression in Solanum lycopersicum hairy roots via Agrobacterium rhizogenes strain 15834 transfection method, and recombinant expression in Pichia pastoris strain X-33	Zea mays
gene ZmNPP2, DNA and amino acid sequence determination and analysis, the gene contains more than one exon, phylogenetic analysis and tree	Zea mays
gene ZmNPP3, DNA and amino acid sequence determination and analysis, the gene contains more than one exon, phylogenetic analysis and tree	Zea mays

Protein Variants

Protein Variants	Comment	Organism
additional information	overexpression of the maize nucleotide pyrophosphatase/phosphodiesterase gene leads to decreased FAD and increased FMN and riboflavin contents in transgenic Arabidopsis thaliana ecotype Col-0 plants. But neither contents nor the ratio of zeatin isomers is altered suggesting that the enzyme is unlikely to catalyze the interconversion of zeatin isomers in vivo	Zea mays

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	the enzyme is secreted	Zea mays	-	-

Organism

Organism	UniProt	Comment	Textmining
Zea mays	A0A1D6NKR9	-	-
Zea mays	A0A1D6PF31	-	-
Zea mays	B6SGJ5	-	-

Subunits

Subunits	Comment	Organism
?	x * 51200, about, sequence calculation, x * 60000, recombinant enzyme expressed from Pichia pastoris strain X-33, SDS-PAGE	Zea mays

Synonyms

Synonyms	Comment	Organism
ectonucleotide pyrophosphatase/phosphodiesterase 1	UniProt	Zea mays
ectonucleotide pyrophosphatase/phosphodiesterase family member 3	UniProt	Zea mays
nucleotide pyrophosphatase/phosphodiesterase	-	Zea mays
ZmNPP1	-	Zea mays
ZmNPP2	-	Zea mays
ZmNPP3	-	Zea mays

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Zea mays

pI Value

Organism	Comment	pI Value Maximum	pI Value
Zea mays	sequence calculation	-	6.36

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Release 2023.1 (January 2023)