Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
mutant E33A, hanging drop vapor diffusion method, using 0.1 Mmagnesium sulfate, 0.1 M MES buffer (pH 6.5), and 10-15% (w/v) PEG 8000 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E33A | inactive | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
dTTP | at pH 7.0 and 25°C | Escherichia coli | |
0.5 | - |
UTP | at pH 7.0 and 25°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P25536 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-charged column chromatography and gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTTP + H2O | the enzyme possesses dTTP-specific activity at a substrate concentration of 0.1 mM | Escherichia coli | dTMP + diphosphate | - |
? | |
dUTP + H2O | weakest substrate | Escherichia coli | dUMP + diphosphate | - |
? | |
additional information | no activity with ATP, CTP, GTP, TDP, TMP, and dCTP | Escherichia coli | ? | - |
? | |
TTP + H2O | the enzyme exhibits significant activity for TTP only when substrate concentration reaches 0.5 mM | Escherichia coli | TMP + diphosphate | - |
? | |
UTP + H2O | the enzyme exhibits significant activity for UTP only when substrate concentration reaches 0.5 mM | Escherichia coli | UMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 45000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
Maf-like protein YhdE | - |
Escherichia coli |
nucleotide pyrophosphatase | - |
Escherichia coli |
PPase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
43 | - |
dTTP | at pH 7.0 and 25°C | Escherichia coli | |
95 | - |
UTP | at pH 7.0 and 25°C | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | enzyme overexpression results in filamentous cells. The enzyme plays a role in in cell growth inhibition under stress conditions, cell-division arrest and cell-shape maintenance | Escherichia coli |
physiological function | emzyme-knockout cells transform the normal rod shape of wild type cells to a more spherical form, and the cell wall appears to become more flexible | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
191 | - |
UTP | at pH 7.0 and 25°C | Escherichia coli | |
503 | - |
dTTP | at pH 7.0 and 25°C | Escherichia coli |