BRENDA - Enzyme Database show
show all sequences of 3.6.1.6

Rat brain nucleoside diphosphatase: purification and properties of type L isoenzyme

Yang, W.J.; Kim, K.W.; Lee, B.J.; Arch. Pharm. Res. 17, 256-262 (1994)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ATP
enhances hydrolysis of GDP
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
diphosphate
0.5 mM, 50% inhibition
Rattus norvegicus
F-
50 mM NaF, more than 80% inhibition
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2
-
GDP
-
Rattus norvegicus
1.4
-
UDP
-
Rattus norvegicus
1.7
-
IDP
-
Rattus norvegicus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
can partially replace Mg2+ in activation
Rattus norvegicus
Mg2+
divalent cation required, maximum activity with Mg2+, half-maximal activity at 0.4 mM MgCl2
Rattus norvegicus
Mn2+
can partially replace Mg2+ in activation
Rattus norvegicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
GDP + H2O
Rattus norvegicus
type L enzyme chiefly works in the degradation of nucleoside diphosphates
GMP + phosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
type L isoenzyme
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
348.4
-
-
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + H2O
no activity
210206
Rattus norvegicus
AMP + phosphate
-
-
-
-
CDP + H2O
-
210206
Rattus norvegicus
CMP + phosphate
-
210206
Rattus norvegicus
?
GDP + H2O
-
210206
Rattus norvegicus
GMP + phosphate
-
210206
Rattus norvegicus
?
GDP + H2O
type L enzyme chiefly works in the degradation of nucleoside diphosphates
210206
Rattus norvegicus
GMP + phosphate
-
-
-
?
IDP + H2O
-
210206
Rattus norvegicus
IMP + phosphate
-
210206
Rattus norvegicus
?
TDP + H2O
-
210206
Rattus norvegicus
TMP + H2O
-
210206
Rattus norvegicus
?
TPP + H2O
-
210206
Rattus norvegicus
thiamine phosphate + phosphate
-
210206
Rattus norvegicus
?
UDP + H2O
-
210206
Rattus norvegicus
UMP + phosphate
-
210206
Rattus norvegicus
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 45000, L-type enzyme, SDS-PAGE
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
9
hydrolysis of GDP
Rattus norvegicus
7.5
8
-
Rattus norvegicus
9
-
hydrolysis of TPP
Rattus norvegicus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.5
-
diphosphate
-
Rattus norvegicus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ATP
enhances hydrolysis of GDP
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
diphosphate
0.5 mM, 50% inhibition
Rattus norvegicus
F-
50 mM NaF, more than 80% inhibition
Rattus norvegicus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.5
-
diphosphate
-
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2
-
GDP
-
Rattus norvegicus
1.4
-
UDP
-
Rattus norvegicus
1.7
-
IDP
-
Rattus norvegicus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
can partially replace Mg2+ in activation
Rattus norvegicus
Mg2+
divalent cation required, maximum activity with Mg2+, half-maximal activity at 0.4 mM MgCl2
Rattus norvegicus
Mn2+
can partially replace Mg2+ in activation
Rattus norvegicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
GDP + H2O
Rattus norvegicus
type L enzyme chiefly works in the degradation of nucleoside diphosphates
GMP + phosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
type L isoenzyme
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
348.4
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + H2O
no activity
210206
Rattus norvegicus
AMP + phosphate
-
-
-
-
CDP + H2O
-
210206
Rattus norvegicus
CMP + phosphate
-
210206
Rattus norvegicus
?
GDP + H2O
-
210206
Rattus norvegicus
GMP + phosphate
-
210206
Rattus norvegicus
?
GDP + H2O
type L enzyme chiefly works in the degradation of nucleoside diphosphates
210206
Rattus norvegicus
GMP + phosphate
-
-
-
?
IDP + H2O
-
210206
Rattus norvegicus
IMP + phosphate
-
210206
Rattus norvegicus
?
TDP + H2O
-
210206
Rattus norvegicus
TMP + H2O
-
210206
Rattus norvegicus
?
TPP + H2O
-
210206
Rattus norvegicus
thiamine phosphate + phosphate
-
210206
Rattus norvegicus
?
UDP + H2O
-
210206
Rattus norvegicus
UMP + phosphate
-
210206
Rattus norvegicus
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 45000, L-type enzyme, SDS-PAGE
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
9
hydrolysis of GDP
Rattus norvegicus
7.5
8
-
Rattus norvegicus
9
-
hydrolysis of TPP
Rattus norvegicus
Other publictions for EC 3.6.1.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733220
Lopez-Esparza
Isolation of Sporothrix schenc ...
Sporothrix schenckii, Sporothrix schenckii EH-206
Arch. Microbiol.
195
499-506
2013
-
-
1
-
-
-
-
-
-
-
1
2
-
6
-
-
-
-
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
733655
Krug
The ATP/ADP substrate specific ...
Toxoplasma gondii
ChemBioChem
14
2292-2300
2013
-
-
-
1
3
-
-
4
-
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
-
-
4
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
4
4
718543
Fausther
Coexpression of ecto-5'-nucleo ...
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
Am. J. Physiol. Gastrointest. Liver Physiol.
302
447-459
2012
-
-
-
-
-
-
-
-
1
2
-
8
-
4
-
-
-
-
-
7
-
-
9
-
1
-
-
-
1
-
-
-
-
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-
-
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-
-
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2
4
-
8
-
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-
9
-
-
9
-
2
-
-
-
2
-
-
-
-
1
2
-
-
-
718541
Lavoie
Ectonucleotidases in the diges ...
Mus musculus, Mus musculus C57BL/6
Am. J. Physiol. Gastrointest. Liver Physiol.
300
G608-G620
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
140
-
-
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8
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1
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1
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8
-
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-
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
720736
Tanaka
Enzymatic activity of the soyb ...
Glycine max
Plant Physiol.
155
1988-1998
2011
-
-
1
-
4
-
1
2
-
2
-
-
-
1
-
-
1
-
-
3
-
-
9
1
-
-
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1
-
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4
-
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1
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2
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2
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1
-
3
-
-
9
1
-
-
-
-
-
-
-
-
-
2
2
-
2
2
711898
Levesque
NTPDase1 governs P2X7-dependen ...
Mus musculus
Eur. J. Immunol.
40
1473-1485
2010
-
-
-
-
-
-
-
-
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1
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1
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1
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2
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2
2
-
-
-
719196
Kauffenstein
The ecto-nucleotidase NTPDase1 ...
Mus musculus 129 SVJ x C57 BL/6, Mus musculus
Br. J. Pharmacol.
159
576-585
2010
-
-
-
-
1
-
1
-
-
1
-
8
-
3
-
-
-
-
-
5
-
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
8
-
-
-
-
-
5
-
-
8
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
698165
Martin-Satue
Localization of plasma membran ...
Mus musculus, Mus musculus C57BL/6, Rattus norvegicus
Histochem. Cell Biol.
131
615-628
2009
-
-
2
-
2
-
-
-
6
2
-
3
-
142
-
-
-
-
-
28
-
-
6
-
2
-
-
-
2
-
-
-
-
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-
2
-
-
2
-
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6
2
-
3
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28
-
-
6
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
669238
Uccelletti
ire-1-dependent transcriptiona ...
Caenorhabditis elegans
J. Biol. Chem.
279
27390-27398
2004
1
-
1
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1
1
3
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4
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2
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2
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1
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1
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1
1
3
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2
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2
-
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670350
Nishiwaki
An NDPase links ADAM protease ...
Caenorhabditis elegans
Nat. Cell Biol.
6
31-37
2004
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-
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1
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1
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3
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3
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654678
Ivanenkov
Bacterial expression,character ...
Homo sapiens
Biochemistry
42
11726-11735
2003
-
-
1
-
1
1
1
1
-
2
1
-
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3
1
1
1
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1
1
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10
1
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1
1
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1
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1
1
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1
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1
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1
1
1
1
1
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-
10
1
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1
1
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656079
Failer
Cloning, expression, and funct ...
Rattus norvegicus
J. Biol. Chem.
277
36978-36986
2002
-
-
1
-
-
-
1
1
2
2
1
1
-
6
-
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1
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8
1
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1
1
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1
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1
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1
2
2
1
1
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1
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8
1
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1
1
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-
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-
656961
Mikami
-
Purification and characterizat ...
Acer pseudoplatanus
Plant Biotechnol.
18
259-265
2001
-
-
-
-
-
-
3
6
1
5
1
-
-
1
-
-
1
-
-
1
2
-
3
1
-
-
1
-
1
-
-
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2
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-
2
3
-
6
1
5
1
-
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1
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1
2
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3
1
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1
-
1
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-
-
-
-
-
-
-
210206
Yang
-
Rat brain nucleoside diphospha ...
Rattus norvegicus
Arch. Pharm. Res.
17
256-262
1994
1
-
-
-
-
-
2
3
-
3
1
1
-
1
-
-
1
-
-
1
1
-
8
1
-
-
-
-
3
-
-
-
1
-
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1
-
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-
-
-
-
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2
1
3
-
3
1
1
-
-
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1
-
1
1
-
8
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
210202
Sano
Type B nucleoside-diphosphatas ...
Rattus norvegicus
Eur. J. Biochem.
171
231-236
1988
-
-
-
-
-
-
10
4
1
8
1
-
-
1
-
-
1
-
-
1
1
-
5
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
4
1
8
1
-
-
-
-
1
-
1
1
-
5
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
210203
De Groot
Loss of latent activity of liv ...
Rattus norvegicus
Biochim. Biophys. Acta
815
91-96
1985
-
1
-
-
-
1
-
-
1
-
-
-
-
2
-
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1
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1
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1
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1
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1
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-
-
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-
-
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-
210204
Sano
Thiamine pyrophosphatase and n ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
118
292-298
1984
1
-
-
-
-
-
2
-
-
-
2
-
-
2
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2
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6
-
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3
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1
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2
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2
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2
-
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6
-
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3
-
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-
-
-
-
-
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-
210205
Ohkubo
Comparative studies on nucleos ...
Rattus norvegicus
Int. J. Biochem.
14
1075-1081
1982
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5
-
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5
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
210207
Brandan
Orientation and role of nucleo ...
Rattus norvegicus
Biochemistry
21
4640-4645
1982
-
-
-
-
-
-
-
-
1
-
-
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-
1
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-
-
-
-
-
-
-
-
-
210208
Ohkubo
Purification and characterizat ...
Rattus norvegicus
Eur. J. Biochem.
112
111-118
1980
-
-
-
-
-
-
3
3
1
1
2
-
-
2
-
1
1
-
-
3
-
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1
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3
-
-
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1
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-
1
-
-
-
-
-
-
-
-
-
210209
Kawakita
Immobilization of nucleoside d ...
Rattus norvegicus
Arch. Biochem. Biophys.
204
326-330
1980
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
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-
1
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1
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2
-
2
-
-
-
-
-
-
-
-
-
-
-
210210
Finch
-
Nucleotide metabolism by micro ...
Oryctolagus cuniculus
Biochem. J.
117
925-930
1979
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
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1
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-
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-
-
-
-
-
-
-
-
-
-
-
-
210211
Kawakita
Allosteric properties of nucle ...
Rattus norvegicus
Biochem. J.
17
3546-3551
1978
2
-
-
-
-
-
-
2
1
-
2
-
-
1
-
-
1
-
-
1
1
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1
-
-
1
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3
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1
-
1
1
-
-
1
-
-
-
3
-
-
-
-
-
-
-
210213
Ishibashi
Affinity purification and some ...
Rattus norvegicus
Biochim. Biophys. Acta
525
265-274
1978
-
-
-
-
-
-
-
1
2
3
2
-
-
2
-
1
1
-
-
2
1
1
5
1
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
1
2
3
2
-
-
-
1
1
-
2
1
1
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210216
Shirasaka
Studies on mitochondrial and m ...
Rattus norvegicus
J. Biochem.
76
99-106
1974
1
-
-
-
-
-
1
-
2
-
2
-
-
1
-
-
1
-
-
1
1
1
6
-
-
-
-
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
2
-
-
-
-
1
-
1
1
1
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210217
Pinsley
Nucleoside diphosphatase from ...
Sus scrofa
Arch. Biochem. Biophys.
158
331-345
1973
-
-
-
-
-
-
-
-
1
-
1
-
-
2
-
-
1
-
-
2
-
-
4
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
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-
1
-
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