BRENDA - Enzyme Database show
show all sequences of 3.6.1.52

Site-directed mutagenesis of diphosphoinositol polyphosphate phosphohydrolase, a dual specificity NUDT enzyme that attacks diadenosine polyphosphates and diphosphoinositol polyphosphates

Yang, X.; Safrany, S.T.; Shears, S.B.; J. Biol. Chem. 274, 35434-35440 (1999)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
E66Q
no significant activity towards diphosphoinositol pentakisphosphate or bisdiphosphoinositol tetrakisphosphate, activity with diadenosine 5',5'''-P1,P6-hexaphosphate is 2% of the activity of the wild-type enzyme
Homo sapiens
E70Q
inactive against dATP,diphosphoinositol pentakisphosphate or bisdiphosphoinositol tetrakisphosphate
Homo sapiens
F84Y
hydrolysis of bisdiphosphoinositol tetrakisphosphate at 63% of the activity of the wild-type enzyme, hydrolysis of diadenosine 5',5'''-P1,P6-hexaphosphate is 75% of the activity of the wild-type enzyme, activity with diphosphoinositol pentakisphosphate is only slightly reduced
Homo sapiens
G50A
hydrolysis of diphosphoinositol polyphosphates is more than 85% less than that of the wild-type enzyme
Homo sapiens
G52A
hydrolysis of diphosphoinositol polyphosphates is more than 85% less than that of the wild-type enzyme
Homo sapiens
G72A
catalytic activity towards bisdiphosphoinositol tetrakisphosphate, diadenosine 5',5'''-P1,P6-hexaphosphateand diphosphoinositol pentakisphosphate is impaired by more than 95%
Homo sapiens
G75A
catalytic activity towards bisdiphosphoinositol tetrakisphosphate, diadenosine 5',5'''-P1,P6-hexaphosphateand diphosphoinositol pentakisphosphate is impaired by more than 95%
Homo sapiens
G78A
catalytic activity and CD spectrum is not significantly different from that of the wild.type enzyme
Homo sapiens
G82A
mutation promotes some unraveling of the normal secondary structure
Homo sapiens
H91L
hydrolysis of bisdiphosphoinositol tetrakisphosphate at 4% of the activity of the wild-type enzyme, hydrolysis of diadenosine 5',5'''-P1,P6-hexaphosphate is 26% of the activity of the wild-type enzyme, activity with diphosphoinositol pentakisphosphate is only slightly reduced
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
recombinant enzyme
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
bisdiphosphoinositol tetrakisphosphate + H2O
-
393826
Homo sapiens
? + phosphate
-
-
-
?
diadenosine 5',5''-P1,P6-hexaphosphate + H2O
-
393826
Homo sapiens
?
-
-
-
?
diphosphoinositol pentakisphosphate + H2O
-
393826
Homo sapiens
inositol pentakisphosphate + phosphate
-
-
-
?
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E66Q
no significant activity towards diphosphoinositol pentakisphosphate or bisdiphosphoinositol tetrakisphosphate, activity with diadenosine 5',5'''-P1,P6-hexaphosphate is 2% of the activity of the wild-type enzyme
Homo sapiens
E70Q
inactive against dATP,diphosphoinositol pentakisphosphate or bisdiphosphoinositol tetrakisphosphate
Homo sapiens
F84Y
hydrolysis of bisdiphosphoinositol tetrakisphosphate at 63% of the activity of the wild-type enzyme, hydrolysis of diadenosine 5',5'''-P1,P6-hexaphosphate is 75% of the activity of the wild-type enzyme, activity with diphosphoinositol pentakisphosphate is only slightly reduced
Homo sapiens
G50A
hydrolysis of diphosphoinositol polyphosphates is more than 85% less than that of the wild-type enzyme
Homo sapiens
G52A
hydrolysis of diphosphoinositol polyphosphates is more than 85% less than that of the wild-type enzyme
Homo sapiens
G72A
catalytic activity towards bisdiphosphoinositol tetrakisphosphate, diadenosine 5',5'''-P1,P6-hexaphosphateand diphosphoinositol pentakisphosphate is impaired by more than 95%
Homo sapiens
G75A
catalytic activity towards bisdiphosphoinositol tetrakisphosphate, diadenosine 5',5'''-P1,P6-hexaphosphateand diphosphoinositol pentakisphosphate is impaired by more than 95%
Homo sapiens
G78A
catalytic activity and CD spectrum is not significantly different from that of the wild.type enzyme
Homo sapiens
G82A
mutation promotes some unraveling of the normal secondary structure
Homo sapiens
H91L
hydrolysis of bisdiphosphoinositol tetrakisphosphate at 4% of the activity of the wild-type enzyme, hydrolysis of diadenosine 5',5'''-P1,P6-hexaphosphate is 26% of the activity of the wild-type enzyme, activity with diphosphoinositol pentakisphosphate is only slightly reduced
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
bisdiphosphoinositol tetrakisphosphate + H2O
-
393826
Homo sapiens
? + phosphate
-
-
-
?
diadenosine 5',5''-P1,P6-hexaphosphate + H2O
-
393826
Homo sapiens
?
-
-
-
?
diphosphoinositol pentakisphosphate + H2O
-
393826
Homo sapiens
inositol pentakisphosphate + phosphate
-
-
-
?
Other publictions for EC 3.6.1.52
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733091
Wu
Elucidating diphosphoinositol ...
Homo sapiens
Angew. Chem. Int. Ed. Engl.
53
7192-7197
2014
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719961
Lonetti
Identification of an evolution ...
Homo sapiens
J. Biol. Chem.
286
31966-31974
2011
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1
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712171
Winward
Oxidation of the diphosphoinos ...
Drosophila melanogaster
Int. J. Biochem. Cell Biol.
42
1174-1181
2010
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-
1
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5
2
4
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1
2
1
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1
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4
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22
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1
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5
1
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1
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5
2
4
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1
1
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4
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22
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1
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5
1
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1
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1
5
5
668226
Chu
Ectopic expression of murine d ...
Mus musculus
Cell. Signal.
16
1045-1059
2004
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1
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1
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1
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7
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7
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1
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657829
Hua
Paralogous murine Nudt10 and N ...
Mus musculus
Biochem. J.
373
81-89
2003
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1
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2
1
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5
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1
1
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1
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1
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1
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1
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1
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5
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659184
Hidaka
An adjacent pair of human NUDT ...
Homo sapiens
J. Biol. Chem.
277
32730-32738
2002
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1
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1
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1
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1
2
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3
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1
1
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2
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8
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2
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1
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2
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8
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659817
Cartwright
The g5R (D250) gene of African ...
African swine fever virus
J. Virol.
76
1415-1421
2002
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1
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1
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2
1
1
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2
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1
1
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1
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1
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1
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1
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1
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1
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1
1
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1
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1
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1
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1
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393827
Caffrey
Discovery of molecular and cat ...
Homo sapiens
J. Biol. Chem.
275
12730-12736
2000
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1
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2
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4
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1
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1
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3
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393825
Safrany
The diadenosine hexaphosphate ...
Homo sapiens
J. Biol. Chem.
274
21735-21740
1999
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2
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4
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393826
Yang
Site-directed mutagenesis of d ...
Homo sapiens
J. Biol. Chem.
274
35434-35440
1999
-
-
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10
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2
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3
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10
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3
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393824
Safrany
A novel context for the 'MutT' ...
Homo sapiens, Rattus norvegicus
EMBO J.
17
6599-6607
1998
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1
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2
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2
2
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1
3
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4
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1
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2
1
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8
1
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2
1
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2
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1
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2
2
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1
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1
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