Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DTT | - |
Daboia russelii | |
EDTA | - |
Daboia russelii | |
iodoacetamide | - |
Daboia russelii | |
additional information | the catalytic activity and platelet deaggregation property of Ruviapyrase is significantly inhibited by EDTA, DTT, and iodoacetamide, and neutralized by commercial monovalent and polyvalent antivenom. Poor inhibition by PMSF | Daboia russelii | |
Zn2+ | 63% (ADPase) and 71% (ATPase) inhibition at 2 mM | Daboia russelii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00254 | - |
ATP | pH 7.4, 37°C | Daboia russelii | |
0.00577 | - |
ADP | pH 7.4, 37°C | Daboia russelii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Daboia russelii | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Daboia russelii | |
Mg2+ | activates, less effective than Ca2+ | Daboia russelii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83041 | - |
MALDI-TOF-mass spectrometry analysis and gel filtration | Daboia russelii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | Daboia russelii | - |
AMP + phosphate | - |
? | |
ATP + H2O | Daboia russelii | - |
ADP + phosphate | - |
? | |
additional information | Daboia russelii | Ruviapyrase does not show cytotoxicity against breast cancer (MCF-7) cells and haemolytic activity, it exhibits marginal anticoagulant and strong antiplatelet activity, and dose-dependently reverses the ADP-induced platelet aggregation. The catalytic activity and platelet deaggregation property of Ruviapyrase is significantly inhibited by EDTA, DTT and IAA, and neutralized by commercial monovalent and polyvalent antivenom | ? | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Daboia russelii | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme is a monomeric glycoprotein that contains 2.4% neutral sugars and 58.4% N-linked oligosaccharides and strongly binds to concanavalin A | Daboia russelii |
Purification (Comment) | Organism |
---|---|
native enzyme 1.6fold (ATPase) and 2.3fold (ADPase) from venom by gel filtration and anion exchange chromatography | Daboia russelii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Daboia russelii | - |
Storage Stability | Organism |
---|---|
the catalytic activity (both ATPase as well as ADPase) of Ruviapyrase declines rapidly at physiological conditions (37°C, pH 7.4). It loses 80% of its enzyme activity within 2 h of incubation albeit ATPase and ADPase activities of crude Russel viper venom are found to be more stable compared to Ruviapyrase | Daboia russelii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | - |
Daboia russelii | AMP + phosphate | - |
? | |
ATP + H2O | - |
Daboia russelii | ADP + phosphate | - |
? | |
additional information | Ruviapyrase does not show cytotoxicity against breast cancer (MCF-7) cells and haemolytic activity, it exhibits marginal anticoagulant and strong antiplatelet activity, and dose-dependently reverses the ADP-induced platelet aggregation. The catalytic activity and platelet deaggregation property of Ruviapyrase is significantly inhibited by EDTA, DTT and IAA, and neutralized by commercial monovalent and polyvalent antivenom | Daboia russelii | ? | - |
- |
|
additional information | Ruviapyrase hydrolysed adenosine triphosphate (ATP) to a significantly greater extent as compared to adenosine diphosphate (ADP). The enzyme is devoid of 5'-nucleotidase and phosphodiesterase activities. The specificity constant or kinetic efficiency of Ruviapyrase in hydrolysing ATP is 3.7folds higher compared to hydrolysis of ADP under identical experimental conditions | Daboia russelii | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
monomer | x * 79400, glycosylated enzyme, SDS-PAGE, x * 33000, deglycosylated enzyme, SDS-PAGE | Daboia russelii |
Synonyms | Comment | Organism |
---|---|---|
Ruviapyrase | - |
Daboia russelii |
snake venom apyrase | - |
Daboia russelii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Daboia russelii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
248.6 | - |
ADP | pH 7.4, 37°C | Daboia russelii | |
410 | - |
ATP | pH 7.4, 37°C | Daboia russelii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Daboia russelii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
43085 | - |
ADP | pH 7.4, 37°C | Daboia russelii | |
161417 | - |
ATP | pH 7.4, 37°C | Daboia russelii |