BRENDA - Enzyme Database show
show all sequences of 3.6.1.39

Thymidine triphosphate nucleotidohydrolase: a phage-induced enzyme in Bacillus subtilis

Roscoe, D.H.; Virology 38, 520-526 (1969)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dTTP + H2O
Bacillus subtilis
-
dTDP + phosphate
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
-
infected with phage phi
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dTTP + H2O
-
210043
Bacillus subtilis
dTMP + diphosphate
-
210043
Bacillus subtilis
?
dTTP + H2O
-
210043
Bacillus subtilis
dTDP + phosphate
-
-
-
-
dUTP + H2O
70% activity compared with dTTP as substrate
210043
Bacillus subtilis
dUMP + diphosphate
-
210043
Bacillus subtilis
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Bacillus subtilis
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
-
-
25% of activity compared with activity at 37°C
Bacillus subtilis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
loss of activity in phage infected cells, no loss of activity in wild type cells
Bacillus subtilis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Bacillus subtilis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.5
10
measured in Tris-buffer and glycine-buffer
Bacillus subtilis
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
9
-
loss of activity above pH 9
Bacillus subtilis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dTTP + H2O
Bacillus subtilis
-
dTDP + phosphate
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dTTP + H2O
-
210043
Bacillus subtilis
dTMP + diphosphate
-
210043
Bacillus subtilis
?
dTTP + H2O
-
210043
Bacillus subtilis
dTDP + phosphate
-
-
-
-
dUTP + H2O
70% activity compared with dTTP as substrate
210043
Bacillus subtilis
dUMP + diphosphate
-
210043
Bacillus subtilis
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Bacillus subtilis
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
-
-
25% of activity compared with activity at 37°C
Bacillus subtilis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
loss of activity in phage infected cells, no loss of activity in wild type cells
Bacillus subtilis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Bacillus subtilis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.5
10
measured in Tris-buffer and glycine-buffer
Bacillus subtilis
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
9
-
loss of activity above pH 9
Bacillus subtilis
Other publictions for EC 3.6.1.39
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719939
Satapathy
The glutamate switch of bacter ...
Enterobacteria phage T7
J. Biol. Chem.
286
23113-23120
2011
1
-
1
-
6
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-
3
-
-
-
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2
-
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-
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2
1
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6
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1
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1
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6
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3
-
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-
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-
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2
1
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-
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6
-
-
-
-
-
-
-
-
4
4
697608
Donmez
Coupling of DNA unwinding to n ...
Enterobacteria phage T7
EMBO J.
27
1718-1726
2008
-
-
-
-
-
-
-
1
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-
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1
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1
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2
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1
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1
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1
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1
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-
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2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
688933
Crampton
DNA-induced switch from indepe ...
Enterobacteria phage T7
Mol. Cell
21
165-174
2006
-
-
-
-
1
-
-
-
-
1
-
1
-
2
-
-
-
-
-
-
-
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2
1
1
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-
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1
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-
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1
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-
-
-
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1
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1
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-
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-
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2
1
1
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1
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-
-
-
-
-
-
-
-
210050
Schultes
Purification and characterizat ...
Homo sapiens
Biol. Chem. Hoppe-Seyler
373
237-247
1992
-
-
-
-
-
-
6
1
-
3
2
1
-
2
-
-
1
-
-
1
-
-
4
1
-
-
-
-
1
1
-
-
4
-
-
-
-
-
-
-
-
-
-
6
4
1
-
3
2
1
-
-
-
1
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1
-
-
4
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
210049
Dahlmann
Serum desoxythymidine-5'-triph ...
Homo sapiens, Mus musculus
Anticancer Res.
11
225-228
1991
-
1
-
-
-
-
-
-
-
-
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2
-
4
-
-
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1
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2
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1
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2
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1
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2
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210051
Schultes
Homogenenous preparation of hu ...
Homo sapiens
Eur. J. Biochem.
192
201-205
1990
-
-
-
-
-
-
-
-
-
-
1
1
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2
-
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1
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1
1
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2
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1
1
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1
1
1
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2
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210045
Dahlmann
A one-step liquid-chromatograp ...
Homo sapiens
Hoppe-Seyler's Z. Physiol. Chem.
365
1263-1269
1984
-
-
-
-
-
-
1
3
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1
1
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2
-
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1
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1
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2
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1
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-
-
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1
1
3
-
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1
1
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1
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1
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2
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-
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-
210044
Dahlman
-
Properties of four different d ...
Homo sapiens
Biochem. Int.
5
185-192
1982
-
-
-
-
-
-
-
1
-
4
-
1
-
1
-
-
1
-
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1
-
1
4
-
-
-
-
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1
1
-
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-
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1
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4
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1
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1
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1
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1
4
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
210046
Dahlmann
Human serum thymidine triphosp ...
Homo sapiens
Biochemistry
21
6634-6639
1982
-
-
-
-
-
-
2
1
-
4
2
1
-
2
-
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1
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2
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3
1
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2
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1
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-
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2
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1
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4
2
1
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1
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2
-
-
3
1
-
-
2
-
1
-
-
-
-
-
-
-
-
-
210047
Dunham
Deoxythymidine triphosphate-de ...
Bacillus subtilis
Biochemistry
13
2667-2672
1974
1
-
-
-
-
-
7
2
1
8
1
1
-
1
-
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1
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-
-
-
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3
-
-
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-
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1
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-
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1
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7
-
2
1
8
1
1
-
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1
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3
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-
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1
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-
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-
-
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-
210048
Price
Thymidine triphosphate nucleot ...
Bacillus subtilis
J. Virol.
10
1240-1241
1972
-
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1
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2
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2
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1
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1
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2
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1
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-
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210043
Roscoe
Thymidine triphosphate nucleot ...
Bacillus subtilis
Virology
38
520-526
1969
-
-
-
-
-
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-
-
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-
-
1
-
2
-
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-
-
-
-
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3
-
1
1
1
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1
1
1
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1
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3
-
1
1
1
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1
1
1
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