Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 12% (w/v) polyethylene glycol 4000, 10% (w/v) 2-propanol and 0.3 M sodium citrate pH 5.65 for the His-tagged enzyme, using 11% (w/v) polyethylene glycol 4000, 10% (w/v) 2-propanol and 0.3 M sodium citrate pH 5.65 for the His-tagged enzyme in complex with dUTP, or using 10% (w/v) polyethylene glycol 4000, 10% (w/v) 2-propanol and 0.3 M sodium citrate pH 5.6 for the mutant enzyme E81S/T84R in complex with dUTP | Chlorella virus |
Protein Variants | Comment | Organism |
---|---|---|
E81S/T84R | the mutations lead to an increase in the optimal temperature of the enzyme to 55°C | Chlorella virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorella virus | - |
- |
- |
Chlorella virus IL-3A | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | - |
Chlorella virus | dUMP + diphosphate | - |
? | |
dUTP + H2O | - |
Chlorella virus IL-3A | dUMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
deoxyuridine triphosphatase | - |
Chlorella virus |
dUTPase | - |
Chlorella virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
wild type enzyme | Chlorella virus |
55 | - |
mutant enzyme E81S/T84R | Chlorella virus |