Cloned (Comment) | Organism |
---|---|
expression as C-terminal domain fusion protein with the nucleocapsid protein in Escherichia coli | Mason-Pfizer monkey virus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant C-terminal domain nucleocapsid fusion enzyme, hanging drop vapour diffusion method, 5 mg/ml protein in 50 mM Tris-HCl, pH 8.0, 0.2 M NH4Cl, 10 mM MgCl2, 5 mM DTT, and 0.5 mM PMSF, with or without 1.3 mM alpha,beta-imino-dUTP, room temperature, mixed 1:1 with reservoir solution containing 0.1 M Tris-HCl, pH 8.5, 8% w/v PEG 8000, X-ray diffraction structure determination and analysis at 1.75-2.3 A resolution | Mason-Pfizer monkey virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mason-Pfizer monkey virus | - |
M-PMV, a beta-retrovirus | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminal domain nucleocapsid fusion protein or free enzyme from Escherichia coli by heparin affinity chromatography and gel filtration | Mason-Pfizer monkey virus |
Subunits | Comment | Organism |
---|---|---|
trimer | homotrimer, crystal structure | Mason-Pfizer monkey virus |
Synonyms | Comment | Organism |
---|---|---|
deoxyuridine 5'-triphosphate nucleotidohydrolase | - |
Mason-Pfizer monkey virus |
dUTPae | - |
Mason-Pfizer monkey virus |