Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxymercuribenzoate | 5 mM, complete inhibition | Homo sapiens | |
EDTA | reversed by Mg2+, Co2+ or Mn2+ | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
dUTP | enzyme from uninfected KB cells | Homo sapiens | |
0.0048 | - |
dUTP | enzyme from herpes simplex virus type 1 infected KB cells | Human alphaherpesvirus 1 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | stimulates, enzyme also exhibits activity in absence of divalent cations | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
enzyme from uninfected KB cells, gel filtration | Homo sapiens |
53000 | - |
enzyme from HSV-1 infected KB cells, gel filtration | Human alphaherpesvirus 1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
HSV-1-induced dUTPase and KB dUTPase | - |
Human alphaherpesvirus 1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa-S3 cell | - |
Homo sapiens | - |
KB cell | KB cells induced or not induced with herpes simplex virus type 1 | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.46 | - |
enzyme from uninfected KB cells | Homo sapiens |
2.171 | - |
enzyme from HSV-1-infected cells | Human alphaherpesvirus 1 |
Storage Stability | Organism |
---|---|
-20°C, 7 days, 2% loss of activity of HSV-1 induced dUTPase | Human alphaherpesvirus 1 |
-20°C, 7 days, 70% loss of activity of uninduced dUTPase | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | - |
Homo sapiens | dUMP + diphosphate | - |
? | |
dUTP + H2O | - |
Human alphaherpesvirus 1 | dUMP + diphosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
t1/2: 35 min for enzyme from HSV-1 infected KB cells | Human alphaherpesvirus 1 |
45 | - |
t1/2: 22 min for enzyme from uninfected KB cells | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | enzyme from HSV-1 infected KB cells | Human alphaherpesvirus 1 |
7 | 8 | enzyme from uninfected KB cells | Homo sapiens |