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Literature summary for 3.6.1.13 extracted from

  • Okuda, K.; Nishiyama, Y.; Morita, E.H.; Hayashi, H.
    Identification and characterization of NuhA, a novel Nudix hydrolase specific for ADP-ribose in the cyanobacterium Synechococcus sp. PCC 7002 (2004), Biochim. Biophys. Acta, 1699, 245-252.
    View publication on PubMed
Search for more literature for 3.6.1.13

Cloned(Commentary)

Cloned (Comment) Organism
-
 Synechococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.094
-
 ADPribose pH 9.5, 37°C Synechococcus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ divalent cation required, Mg2+ is most effective Synechococcus sp.
Mn2+ divalent cation required, Mn2+ results in 22% of the activity observed in presence of Mg2+ Synechococcus sp.
Zn2+ divalent cation required, Zn2+ results in 9% of the activity observed in presence of Mg2+ Synechococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26735
-
 6 * 26735, calculation from nucleotide sequence Synechococcus sp.
28000
-
 6 * 28000, SDS-PAGE Synechococcus sp.
166000
-
 gel filtration Synechococcus sp.

Organism

Organism UniProt Comment Textmining
Synechococcus sp. Q83ZD0 PCC 7002
-

Purification (Commentary)

Purification (Comment) Organism
-
 Synechococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-glucose + H2O 9% of the activity with ADPribose Synechococcus sp. AMP + alpha-D-glucose 1-phosphate
-
 ?
ADPribose + H2O
-
 Synechococcus sp. AMP + D-ribose 5-phosphate
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 28000, SDS-PAGE Synechococcus sp.
hexamer 6 * 26735, calculation from nucleotide sequence Synechococcus sp.

Synonyms

Synonyms Comment Organism
NuhA
-
 Synechococcus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8
-
 ADPribose pH 9.5, 37°C Synechococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Synechococcus sp.

pH Range

pH Minimum pH Maximum Comment Organism
8 10 pH 8.0: about 40% of maximal activity, pH 10.0: about 60% of maximal activity Synechococcus sp.

pI Value

Organism Comment pI Value Maximum pI Value
Synechococcus sp.
-
-
 6