Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 45% inhibition of polyphosphatase I and 55% inhibition of polyphosphatase II, both at 0.3 mM | Microlunatus phosphovorus | |
heparin | complete inhibition of polyphosphatase I at 0.05 mg/ml, 35% inhibition of polyphosphatase II at 0.1 mg/ml | Microlunatus phosphovorus | |
additional information | NaF and vanadate are not inhibitors | Microlunatus phosphovorus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates 4.4fold polyphosphatase II and activates 1.5fold polyphosphatase I both at 0.15 mM | Microlunatus phosphovorus | |
K+ | activates 2fold polyphosphatase II, but not activates polyphosphatase I | Microlunatus phosphovorus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
polyphosphatase II, gel filtration | Microlunatus phosphovorus |
93000 | - |
polyphosphatase I, gel filtration | Microlunatus phosphovorus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Microlunatus phosphovorus | - |
NM-1 strain, two isoenzymes polyphosphatases I and II | - |
Purification (Comment) | Organism |
---|---|
isolation of two isoenzymes by cellular fractionation and gel filtration | Microlunatus phosphovorus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(polyphosphate)n + H2O | polyphosphatase I shows optimal activity when n is 25 phosphate residues, polyphosphatases II prefers substrates with 9 residues | Microlunatus phosphovorus | (polyphosphate)n-1 + phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
polyphosphatase I | Microlunatus phosphovorus |
7.5 | - |
polyphosphatase II | Microlunatus phosphovorus |