Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GSH | activates up to 4 mM, and inhibits at higher concentrations | Helicobacter pylori | |
NaBH4 | activates at above 12 mM | Helicobacter pylori |
Protein Variants | Comment | Organism |
---|---|---|
C16S | site-directed mutagenesis, the mutant shows a loss of 50% activity and a reduction in sensitivity to reductants and oxidized glutathione compared to the wild-type enzyme. In addition, the replacement causes a considerable disruption in thermostability, C16S substitution destabilizes PPase through impairing trimer-trimer interactions | Helicobacter pylori |
D69G | site-directed mutagenesis, inactive mutant | Helicobacter pylori |
K103A | site-directed mutagenesis, inactive mutant | Helicobacter pylori |
Y140F | site-directed mutagenesis, the mutant shows 78% reduced activity compared to the wild-type enzyme | Helicobacter pylori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GSH | activates up to 4 mM, and inhibits at higher concentrations | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | effects of cysteine, homocysteine, and NaBH4 on kinetics of wild-type and mutant C16S enzymes, overview | Helicobacter pylori | |
0.083 | - |
diphosphate | pH 8.0, 37°C, recombinant wild-type enzyme | Helicobacter pylori | |
0.098 | - |
diphosphate | pH 8.0, 37°C, recombinant mutant C16S | Helicobacter pylori | |
0.173 | - |
diphosphate | pH 8.0, 37°C, recombinant mutant Y140F | Helicobacter pylori |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Helicobacter pylori | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on, other divalent cations substituted poorly for Mg2+ | Helicobacter pylori | |
additional information | no effect by Ca2+, Cd2+, and Ni2+ | Helicobacter pylori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
6 * 20000 | Helicobacter pylori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Helicobacter pylori | - |
2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | P56153 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Helicobacter pylori | 2 phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 20000 | Helicobacter pylori |
Synonyms | Comment | Organism |
---|---|---|
inorganic pyrophosphatase | - |
Helicobacter pylori |
More | Helicobacter pylori is a family I PPase | Helicobacter pylori |
PPase | - |
Helicobacter pylori |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Helicobacter pylori |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
residue Cys116 is involved in thermostability | Helicobacter pylori |
37 | - |
15 min, the mutant enzyme loses 70% activity in absence of Mg2+, half-life is 4.2 min | Helicobacter pylori |
74.3 | - |
T1/2 of the wild-type enzyme in absence of Mg2+ | Helicobacter pylori |
77.6 | - |
T1/2 of the wild-type enzyme in presence of Mg2+ | Helicobacter pylori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
78.4 | - |
diphosphate | pH 8.0, 37°C, recombinant mutant Y140F | Helicobacter pylori | |
173.1 | - |
diphosphate | pH 8.0, 37°C, recombinant mutant C16S | Helicobacter pylori | |
343.9 | - |
diphosphate | pH 8.0, 37°C, recombinant wild-type enzyme | Helicobacter pylori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Helicobacter pylori |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
17.2 | - |
pH 8.0, 37°C, recombinant wild-type enzyme | Helicobacter pylori | GSH |