Protein Variants | Comment | Organism |
---|---|---|
D102V | large decrease in metal binding affinity | Escherichia coli |
D65E | large decrease in metal binding affinity | Escherichia coli |
D70E | strongly decreased affinity for diphosphate | Escherichia coli |
D97E | increases affinity for diphosphate | Escherichia coli |
E20D | large decrease in metal binding affinity | Escherichia coli |
E20D | strongly decreased affinity for diphosphate | Escherichia coli |
E31D | increased metal binding affinity | Escherichia coli |
E31D | strongly decreased affinity for diphosphate | Escherichia coli |
H136Q | increased metal binding affinity | Escherichia coli |
K29R | strongly decreased affinity for diphosphate | Escherichia coli |
additional information | several mutants described that have minor effects on the binding of metal ions or diphosphate | Escherichia coli |
R43K | strongly decreased affinity for diphosphate | Escherichia coli |
Y55F | increased metal binding affinity | Escherichia coli |
Y55F | strongly decreased affinity for diphosphate | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydroxymethylbisphosphonate | competitive with diphosphate | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | type I | Escherichia coli | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, three binding sites in the absence of substrate | Escherichia coli | |
Mn2+ | can partially replace Mg2+ | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Escherichia coli | important for energy metabolism, provides energy for biosynthetic reactions | 2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Escherichia coli | 2 phosphate | - |
? | |
diphosphate + H2O | important for energy metabolism, provides energy for biosynthetic reactions | Escherichia coli | 2 phosphate | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
hydroxymethylbisphosphonate | pH 7.2 | Escherichia coli |