General Stability | Organism |
---|---|
decreasing activity at 4°C in reaction mixture lacking diphosphate , KCl prevents from inactivation, Mg-diphosphate, does not protect from inactivation | Methanothermobacter thermautotrophicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KF | 50% at 0.9 mM | Methanothermobacter thermautotrophicus | |
Mn2+ | 60% inhibition at 1 mM Mn2+: 24 mM Mg2+ | Methanothermobacter thermautotrophicus | |
NaF | - |
Methanothermobacter thermautotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
diphosphate | - |
Methanothermobacter thermautotrophicus | |
4.9 | - |
Mg2+ | - |
Methanothermobacter thermautotrophicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Methanothermobacter thermautotrophicus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can partially replace Mg2+, 15% | Methanothermobacter thermautotrophicus | |
Mg2+ | required for activity | Methanothermobacter thermautotrophicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
4 * 25000, gel filtration, dimeric form also active | Methanothermobacter thermautotrophicus |
100000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Methanothermobacter thermautotrophicus | - |
2 phosphate | - |
? | |
diphosphate + H2O | Methanothermobacter thermautotrophicus H | - |
2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Methanothermobacter thermautotrophicus H | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanothermobacter thermautotrophicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1233 | - |
- |
Methanothermobacter thermautotrophicus |
Storage Stability | Organism |
---|---|
-20°C, Tris-HCl, one year, negligible loss of activity | Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Methanothermobacter thermautotrophicus | 2 phosphate | - |
? | |
diphosphate + H2O | high specificity | Methanothermobacter thermautotrophicus | 2 phosphate | - |
? | |
diphosphate + H2O | - |
Methanothermobacter thermautotrophicus H | 2 phosphate | - |
? | |
diphosphate + H2O | high specificity | Methanothermobacter thermautotrophicus H | 2 phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 25000, gel filtration, dimeric form also active | Methanothermobacter thermautotrophicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Methanothermobacter thermautotrophicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
60°C | Methanothermobacter thermautotrophicus |