Cloned (Comment) | Organism |
---|---|
expression of C-terminally Strep-tagged TenA in Escherichia coli strain BL21(DE3) | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant C-terminally Strep-tagged TenA, hanging drop vapour diffusion method, 0.002 ml of 10 mg/ml protein in 100 mM Tris-HCl buffer pH 8.0, is mixed with 0.002 l of reservoir solution containing 0.2 M sodium acetate, 0.1 M Tris-HCl pH 8.5 and 28% w/v PEG 3350, 20°C, 7 days, X-ray diffraction structure determination and analysis at 2.60 A resolution, molecular replacement, modelling | Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26840 | - |
x * 26840, sequence calculation | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally Strep-tagged TenA from Escherichia coli strain BL21(DE3) by affinity chromatography | Staphylococcus aureus |
Subunits | Comment | Organism |
---|---|---|
? | x * 26840, sequence calculation | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
TenA | - |
Staphylococcus aureus |
thiaminase type II | - |
Staphylococcus aureus |
General Information | Comment | Organism |
---|---|---|
metabolism | thiaminase type II, TenA, catalyzes the deamination of aminopyrimidines, including the cleavage of thiamine to 4-amino-5-hydroxymethyl-2-methylpyrimidine and 5-(2-hydroxyethyl)-4-methylthiazole in the metabolism of thiamine (vitamin B1), in Staphylococcus aureus | Staphylococcus aureus |
additional information | putative active-site residues are Asp44, Cys137, Tyr167 and Glu208 | Staphylococcus aureus |