Cloned (Comment) | Organism |
---|---|
gene ridA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21, quantitative expression analysis in Bgl- and Bgl+ strain ZK819 derivatives, overview | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AF93 | two Bgl- strains ZK819-Tn10 and ZK819-Tn5 are isogenic derivatives of a common Bgl- parent strain ZK819 differing only in their antibiotic resistance markers that have been verified to be neutral. The two Bgl+ strains ZK819-97T and ZK819-bglR::IS1 have also been derived from the same Bgl- parent strain ZK819. The two strains differ only in the nature of the mutation that activates the operon. While ZK819-97T carries a point mutation in the CAP-cAMP binding site of the operon, ZK819-bglR::IS1 carries an IS1 insertion in the bglR regulatory region that activates the operon | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and dialysis | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
ridA | - |
Escherichia coli |
yjgF | - |
Escherichia coli |
Organism | Comment | Expression |
---|---|---|
Escherichia coli | leucine antagonizes the activation by inducing transcriptional factor Lrp | down |
Escherichia coli | the ridA gene of Escherichia coli is indirectly regulated by BglG through the transcriptional regulator Lrp in stationary phase. Gene ridA is positively regulated by leucine responsive regulatory protein (Lrp) and leucine antagonizes the activation by Lrp, putative Lrp binding site within the ridA promoter. Purified Lrp protein binds to ridA promoter in vitro | up |
General Information | Comment | Organism |
---|---|---|
malfunction | loss of ridA function in a Bgl+ background results in a significant growth retardation in serine-containing media compared to that in a Bgl- background. Deletion of ridA is more disadvantageous in a Bgl+ background, complex metabolic phenotypes like sensitivity to serine in glucose-rich medium and inability to grow on pyruvate as the sole carbon source, overview | Escherichia coli |
metabolism | the ridA gene of Escherichia coli is indirectly regulated by BglG through the transcriptional regulator Lrp in stationary phase | Escherichia coli |
physiological function | reactive metabolites of enamine/imine nature generated during the breakdown of amino acids like serine and threonine are highly nucleophilic and pose a serious threat to cell viability. RidA deaminates these metabolites and facilitates their conversion into utilizable products, thus preventing cellular damage. Overexpression of ridA in Bgl+ background during stationary phase is physiologically relevant to eliminate toxic metabolites generated by the catabolism of serine-containing peptides as a result of elevated levels of their uptake | Escherichia coli |