Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.5.4 extracted from

  • Acera, F.; Carmona, M.I.; Castillo, F.; Quesada, A.; Blasco, R.
    A cyanide-induced 3-cyanoalanine nitrilase in the cyanide-assimilating bacterium Pseudomonas pseudoalcaligenes strain CECT 5344 (2017), Appl. Environ. Microbiol., 83, e00089-17 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.5.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli enables the recombinant strain to fully assimilate 3-cyanoalanine Pseudomonas oleovorans

Organism

Organism UniProt Comment Textmining
Pseudomonas oleovorans W6R265
-
-
Pseudomonas oleovorans CECT 5344 W6R265
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas oleovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-cyano-L-alanine + 2 H2O
-
 Pseudomonas oleovorans L-aspartate + NH3
-
 ?
3-cyano-L-alanine + 2 H2O
-
 Pseudomonas oleovorans CECT 5344 L-aspartate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
3-cyanoalanine nitrilase
-
 Pseudomonas oleovorans
Nit4
-
 Pseudomonas oleovorans

Expression

Organism Comment Expression
Pseudomonas oleovorans cyanide as a sole nitrogen source induces activity up

General Information

General Information Comment Organism
malfunction a nit4 knockout mutant of Pseudomonas pseudoalcaligenes CECT 5344 becomes severely impaired in its ability to grow with 3-cyanoalanine and cyanide as nitrogen sources Pseudomonas oleovorans
metabolism the gene cluster including cio1 and nit4 could be involved both in cyanide resistance and in its assimilation by Pseudomonas pseudoalcaligenes CECT 5344 Pseudomonas oleovorans