Cloned (Comment) | Organism |
---|---|
cloned into expression vector pET-28a and expressed in Escherichia coli strain BL21(DE3) | Rhodococcus rhodochrous |
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) under the control of T7 lac promoter using pET-28a to give pET-Nit, lactose is best as inducer in different media, method optimization | Rhodococcus rhodochrous |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
SDS-PAGE | Rhodococcus rhodochrous |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus rhodochrous | A4LA85 | - |
- |
Rhodococcus rhodochrous tg1-A6 | A4LA85 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-cyanopyridine + 2 H2O | about 50% activity compared to acrylonitrile | Rhodococcus rhodochrous | nicotinic acid + NH3 | - |
? | |
3-cyanopyridine + 2 H2O | about 50% activity compared to acrylonitrile | Rhodococcus rhodochrous tg1-A6 | nicotinic acid + NH3 | - |
? | |
3-cyanopyridine + 2 H2O | 50% of the activity with acrylonitrile | Rhodococcus rhodochrous | pyridine 3-carboxylic acid + NH3 | - |
? | |
3-cyanopyridine + 2 H2O | 50% of the activity with acrylonitrile | Rhodococcus rhodochrous tg1-A6 | pyridine 3-carboxylic acid + NH3 | - |
? | |
acrylonitrile + 2 H2O | best substrate | Rhodococcus rhodochrous | acrylic acid + NH3 | - |
? | |
acrylonitrile + H2O | 100% activity, the optimum substrate concentration is 225 mM acrylonitrile | Rhodococcus rhodochrous | acrylic acid + NH3 | - |
? | |
acrylonitrile + H2O | 100% activity, the optimum substrate concentration is 225 mM acrylonitrile | Rhodococcus rhodochrous tg1-A6 | acrylic acid + NH3 | - |
? | |
aminoacetonitrile + 2 H2O | 25% of the activity with acrylonitrile | Rhodococcus rhodochrous | aminoacetic acid + NH3 | - |
? | |
aminoacetonitrile + H2O | about 30% activity compared to acrylonitrile | Rhodococcus rhodochrous | ? | - |
? | |
glycolonitrile + 2 H2O | 40% of the activity with acrylonitrile | Rhodococcus rhodochrous | glycolic acid + NH3 | - |
? | |
glycolonitrile + H2O | about 40% activity compared to acrylonitrile | Rhodococcus rhodochrous | ? | - |
? | |
iminodiacetonitrile + 2 H2O | about 25% activity compared to acrylonitrile | Rhodococcus rhodochrous | iminodiacetic acid + 2 NH3 | - |
? | |
iminodiacetonitrile + 2 H2O | 20% of the activity with acrylonitrile | Rhodococcus rhodochrous | iminodiacetic acid + 2 NH3 | - |
? | |
iminodiacetonitrile + 2 H2O | about 25% activity compared to acrylonitrile | Rhodococcus rhodochrous tg1-A6 | iminodiacetic acid + 2 NH3 | - |
? | |
iminodiacetonitrile + 2 H2O | 20% of the activity with acrylonitrile | Rhodococcus rhodochrous tg1-A6 | iminodiacetic acid + 2 NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE | Rhodococcus rhodochrous |
? | x * 42000, recombinant enzyme, SDS-PAGE | Rhodococcus rhodochrous |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rhodococcus rhodochrous |
45 | - |
recombinant nitrilase | Rhodococcus rhodochrous |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Rhodococcus rhodochrous |
8 | - |
recombinant nitrilase | Rhodococcus rhodochrous |