General Stability | Organism |
---|---|
stabilized by 2-mercaptoethanol, labile to heat | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-aminoimidazole-4-carboxamide | - |
Oryctolagus cuniculus | |
5-aminoimidazole-4-carboxamide | - |
Rattus norvegicus | |
5-aminoimidazole-4-carboxamide ribonucleoside | - |
Oryctolagus cuniculus | |
5-aminoimidazole-4-carboxamide ribonucleoside | - |
Rattus norvegicus | |
iodoacetamide | - |
Oryctolagus cuniculus | |
iodoacetic acid | - |
Oryctolagus cuniculus | |
p-hydroxymercuribenzoate | - |
Oryctolagus cuniculus | |
p-hydroxymercuribenzoate | - |
Rattus norvegicus | |
tetrahydrofolic acid | - |
Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0125 | - |
guanine | - |
Oryctolagus cuniculus | |
0.0182 | - |
guanine | buffer with 50% D2O | Oryctolagus cuniculus | |
0.025 | - |
guanine | buffer with 100% D2O | Oryctolagus cuniculus | |
0.333 | - |
8-Azaguanine | at pH 6 | Oryctolagus cuniculus | |
0.8 | - |
6-thioguanine | - |
Oryctolagus cuniculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
SDS-PAGE | Oryctolagus cuniculus |
55000 | - |
liver, single polypeptide chain, gel filtration | Rattus norvegicus |
55000 | - |
liver, single polypeptide chain, gel filtration | Oryctolagus cuniculus |
56000 | - |
liver enzyme, gel filtration | Oryctolagus cuniculus |
170000 | - |
- |
Oryctolagus cuniculus |
525000 | - |
- |
Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + H2O | Rattus norvegicus | - |
xanthine + NH3 | - |
? | |
guanine + H2O | Oryctolagus cuniculus | - |
xanthine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
rabbit | - |
Rattus norvegicus | - |
rat | - |
Purification (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
liver | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25.2 | - |
liver enzyme | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-thioguanine + H2O | - |
Oryctolagus cuniculus | 6-thioxanthine + NH3 | - |
? | |
8-azaguanine + H2O | - |
Oryctolagus cuniculus | 8-azaxanthine + NH3 | - |
? | |
guanine + H2O | - |
Rattus norvegicus | xanthine + NH3 | - |
? | |
guanine + H2O | - |
Oryctolagus cuniculus | xanthine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Rattus norvegicus |
monomer | 1 * 55000, gel filtration, SDS-PAGE | Oryctolagus cuniculus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | activation energy linear | Oryctolagus cuniculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Rattus norvegicus |
50 | - |
relatively stable up to 50°C | Oryctolagus cuniculus |
55 | - |
42% loss of enzyme activity substained upon incubation for 25 min | Oryctolagus cuniculus |
55 | - |
loss of catalytic activity upon incubation for 20 min | Oryctolagus cuniculus |
60 | - |
- |
Rattus norvegicus |
60 | - |
irreversible denaturation upon incubation for 25 min | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
8-azaguanine as substrate | Oryctolagus cuniculus |
6.8 | - |
pH optimum is independent of the buffer system | Oryctolagus cuniculus |
7.7 | - |
- |
Oryctolagus cuniculus |
8 | - |
- |
Rattus norvegicus |
8 | - |
- |
Oryctolagus cuniculus |