Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dCTP | enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster: maximum activation is achieved by lower concentration of dCTP than of enzyme from non-infected cells | Herpes simplex virus | |
dCTP | enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster: maximum activation is achieved by lower concentration of dCTP than of enzyme from non-infected cells | Cricetinae | |
Herpes antiserum | activation of host-cell enzyme | Cricetinae | |
additional information | no activation by KCl up to 0.3 M | Herpes simplex virus | |
additional information | no activation by KCl up to 0.3 M | Cricetinae | |
pre-immune serum | activation of host-cell enzyme, twice as effective as Herpes antiserum | Cricetinae |
General Stability | Organism |
---|---|
dCTP and Mg2+ stabilize | Cricetinae |
enzyme very sensitive to preliminary heating, dCTP, 0.125 mM, and Mg2+, 2 mM, stabilize against heat inactivation | Herpes simplex virus |
enzyme very sensitive to preliminary heating, dCTP, 0.125 mM, and Mg2+, 2 mM, stabilize against heat inactivation | Cricetinae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dTTP | enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster is more resistant to inhibition by dTTP than enzyme from non-infected cells | Cricetinae | |
dTTP | enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster is more resistant to inhibition by dTTP than enzyme from non-infected cells | Herpes simplex virus | |
Herpes antiserum | inhibition of Herpes infected-cell enzyme | Herpes simplex virus | |
additional information | no inhibition by KCl up to 0.3 M | Cricetinae | |
additional information | no inhibition by KCl up to 0.3 M | Herpes simplex virus | |
pre-immune serum | inhibition of Herpes infected-cell enzyme | Herpes simplex virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
dCMP | in the presence of dCTP and presence of 2 mM Mg2+ | Herpes simplex virus | |
0.32 | - |
dCMP | in the presence of dCTP and presence of 2 mM Mg2+ | Cricetinae | |
0.91 | - |
dCMP | in the presence of dCTP and absence of 2 mM Mg2+ | Herpes simplex virus | |
0.91 | - |
dCMP | in the presence of dCTP and absence of 2 mM Mg2+ | Cricetinae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for complete activation by dCTP | Herpes simplex virus | |
Mg2+ | required for complete activation by dCTP | Cricetinae | |
Mg2+ | required for activation by dCTP and for inhibition by dTTP | Herpes simplex virus | |
Mg2+ | required for activation by dCTP and for inhibition by dTTP | Cricetinae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dCMP + H2O | Herpes simplex virus | enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis | dUMP + NH3 | - |
? | |
dCMP + H2O | Cricetinae | enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis | dUMP + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cricetinae | - |
BHK-21/C13 cells, baby-hamster, enzyme from non-infected cells and cells infected by Herpes simplex | - |
Herpes simplex virus | - |
induced by | - |
Herpes simplex virus | - |
host baby-hamster, BHK-21/C13 cells | - |
Purification (Comment) | Organism |
---|---|
BHK-21/C13 cells , enzyme from non-infected cells and cells infected by Herpes simplex | Cricetinae |
host baby-hamster, BHK-21/C13 cells | Herpes simplex virus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
BHK-21 cell | baby-hamster kidney | Cricetinae | - |
kidney | BHK-21/C13 cells, baby-hamster kidney cells | Cricetinae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dCMP + H2O | the dCMP concentration at half-maximum velocity is lower by enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster than by enzyme from non-infected cells | Herpes simplex virus | dUMP + NH3 | - |
? | |
dCMP + H2O | the dCMP concentration at half-maximum velocity is lower by enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster than by enzyme from non-infected cells | Cricetinae | dUMP + NH3 | - |
? | |
dCMP + H2O | enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis | Herpes simplex virus | dUMP + NH3 | - |
? | |
dCMP + H2O | enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis | Cricetinae | dUMP + NH3 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Herpes simplex virus |
37 | - |
assay at | Cricetinae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme very sensitive to preliminary heating, dCTP, 0.125 mM, and Mg2+, 2 mM, stabilizes against heat inactivation. Host-cell enzyme is more sensitive to heating than Herpes virus infected enzyme | Herpes simplex virus |
additional information | - |
enzyme very sensitive to preliminary heating, dCTP, 0.125 mM, and Mg2+, 2 mM, stabilizes against heat inactivation. Host-cell enzyme is more sensitive to heating than Herpes virus infected enzyme | Cricetinae |
37 | - |
enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster is more resistant to heating at 37°C than enzyme from non-infected cells. In the presence of 0.125 mM dCTP and 2 mM Mg2+, 100% stable, 20 min at 37°C | Herpes simplex virus |
37 | - |
enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster is more resistant to heating at 37°C than enzyme from non-infected cells. In the presence of 0.125 mM dCTP and 2 mM Mg2+, 100% stable, 20 min at 37°C | Cricetinae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme from host-cell and Herpes virus-induced enzyme have a similar pH-optimum | Herpes simplex virus |
additional information | - |
enzyme from host-cell and Herpes virus-induced enzyme have a similar pH-optimum | Cricetinae |