Cloned (Comment) | Organism |
---|---|
phylogenetic tree, overview | Paracoccus sp. WB1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete inactivation at 1 mM | Paracoccus sp. WB1 | |
Co2+ | 50% inhibition at 1 mM | Paracoccus sp. WB1 | |
Cu2+ | complete inactivation at 1 mM | Paracoccus sp. WB1 | |
Hg2+ | complete inactivation at 1 mM | Paracoccus sp. WB1 | |
additional information | NaN3, and chelating agents EDTA and 1,10-phenanthroline do not inhibit the activity at the concentration of 20 mM, no inhibition by Tween80 and SDS, and by Ca2+, Pb2+, Ba2+, Fe2+, Fe3+, Mg2+, Cr2+, Mn2+, and Li+ | Paracoccus sp. WB1 | |
Tween20 | strong inhibition at 1 mM | Paracoccus sp. WB1 | |
Zn2+ | 40% inhibition at 1 mM | Paracoccus sp. WB1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
Creatine | pH 7.5, 37°C | Paracoccus sp. WB1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
x * 48000, SDS-PAGE | Paracoccus sp. WB1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
creatine + H2O | Paracoccus sp. WB1 | - |
sarcosine + urea | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paracoccus sp. WB1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 10.17fold to homogeneity by anion exchange chromatography, hydrophobic interaction chromatography, and gel filtration | Paracoccus sp. WB1 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
measurement of creatinine in a coupled assay with creatininase and sarcosine oxidase, overview | Paracoccus sp. WB1 |
6.1 | - |
purified enzyme | Paracoccus sp. WB1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
creatine + H2O | - |
Paracoccus sp. WB1 | sarcosine + urea | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48000, SDS-PAGE | Paracoccus sp. WB1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Paracoccus sp. WB1 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | - |
Paracoccus sp. WB1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | purified enzyme, stable | Paracoccus sp. WB1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Paracoccus sp. WB1 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | - |
Paracoccus sp. WB1 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | purified enzyme, stable | Paracoccus sp. WB1 |