Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptopropionate | 5 mM, isoform LeARG1, 38% residual activity, LeARG2, 38% residual activity; 5 mM, isoform LeARG1, 38% residual activity, LeARG2, 38% residual activity | Solanum lycopersicum | |
3-Mercaptopropionate | 5 mM, isoform LeARG1, 24% residual activity, LeARG2, 26% residual activity; 5 mM, isoform LeARG1, 24% residual activity, LeARG2, 26% residual activity | Solanum lycopersicum | |
Mercaptoacetate | 5 mM, isoform LeARG1, 28% residual activity, LeARG2, 26% residual activity; 5 mM, isoform LeARG1, 28% residual activity, LeARG2, 26% residual activity | Solanum lycopersicum | |
Nomega-hydroxy-L-arginine | 0.2 mM, isoform LeARG1, 7% residual activity, LeARG2, 5% residual activity; 0.2 mM, isoform LeARG1, 7% residual activity, LeARG2, 5% residual activity | Solanum lycopersicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, SDS-PAGE and calculated | Solanum lycopersicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | Q5UNS1 | isoform LeARG2; isoform LeARG2 | - |
Solanum lycopersicum | Q5UNS2 | isoform LeARG1; enzyme isoform LeARG1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | induction of isoform LeARG2 upon wounding and treatment with jasmonic acid | Solanum lycopersicum | - |
additional information | highest levels of enzyme activity in reproductive tissues | Solanum lycopersicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agmatine + H2O | isoform LeARG1, 0.5% of the activity with L-arginine, LeARG2, 0.6% of the activity with L-arginine | Solanum lycopersicum | 1,4-diaminobutane + urea | - |
? | |
D-arginine + H2O | isoform LeARG1, 2.2% of the activity with L-arginine, LeARG2, 3.6% of the activity with L-arginine | Solanum lycopersicum | D-ornithine + urea | - |
? | |
L-arginine + H2O | - |
Solanum lycopersicum | L-ornithine + urea | - |
? | |
L-homoarginine + H2O | isoform LeARG1, 14% of the activity with L-arginine, LeARG2, 13% of the activity with L-arginine | Solanum lycopersicum | L-2,6-diaminohexanoate + urea | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, SDS-PAGE and calculated | Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Solanum lycopersicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 12 | no activity below or above this range | Solanum lycopersicum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
Nomega-hydroxy-L-arginine | pH 9.6, 37°C | Solanum lycopersicum |