Literature summary for 3.5.1.99 extracted from

Lodola, A.; Mor, M.; Hermann, J.C.; Tarzia, G.; Piomelli, D.; Mulholland, A.J.
QM/MM modelling of oleamide hydrolysis in fatty acid amide hydrolase (FAAH) reveals a new mechanism of nucleophile activation (2005), Chem. Commun. (Camb. ), 35, 4399-401.

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Application

Application	Comment	Organism
medicine	FAAH is a promising target for the treatment of several central and peripheral nervous system disorders, such as anxiety, pain and hypertension	Rattus norvegicus

Protein Variants

Protein Variants	Comment	Organism
K142A	the greater reduction of Ser241 labelling rate in the K142A/S217A double mutant, compared to the K142A and S217A single mutants, suggests that Lys142 and Ser217 cooperate to deprotonate Ser241	Rattus norvegicus
K142A/S217A	the greater reduction of Ser241 labelling rate in the K142A/S217A double mutant, compared to the K142A and S217A single mutants, suggests that Lys142 and Ser217 cooperate to deprotonate Ser241	Rattus norvegicus
S217A	the greater reduction of Ser241 labelling rate in the K142A/S217A double mutant, compared to the K142A and S217A single mutants, suggests that Lys142 and Ser217 cooperate to deprotonate Ser241	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	hybrid quantum mechanics/molecular mechanics (QM/MM) calculations reveal a new mechanism of nucleophile activation involving a LysSerSer catalytic triad. The proposed mechanism, shows that Lys142 and cis-Ser217 have a direct role in the activation of Ser241, in agreement with kinetic labelling experiments employing the highly reactive fluorophosphonatetetramethyl rhodamine. The greater reduction of Ser241 labelling rate in the K142A/S217A double mutant, compared to the K142A and S217A single mutants, suggests that Lys142 and Ser217 cooperate to deprotonate Ser241	Rattus norvegicus	?	-	?

Synonyms

Synonyms	Comment	Organism
FAAH	-	Rattus norvegicus

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Release 2023.1 (January 2023)