Literature summary for 3.5.1.77 extracted from

Yu, H.; Li, J.; Zhang, D.; Yang, Y.; Jiang, W.; Yang, S.
Improving the thermostability of N-carbamyl-D-amino acid amidohydrolase by error-prone PCR (2009), Appl. Microbiol. Biotechnol., 82, 279-285.

Application

Application	Comment	Organism
biotechnology	the isolated DCase enzyme, with improved thermostability and solubility, is expected to be used in the development of a fully enzymatic process for the industrial production of D-amino acids	Ralstonia pickettii

Cloned(Commentary)

Cloned (Comment)	Organism
into the vector pET28b, a mutagenesis library is created	Ralstonia pickettii

Protein Variants

Protein Variants	Comment	Organism
A164T	mutant, screening for thermostable enzyme	Ralstonia pickettii
A36E	mutant, screening for thermostable enzyme	Ralstonia pickettii
A36V	mutant, screening for thermostable enzyme	Ralstonia pickettii
H248Q	mutant, screening for thermostable enzyme	Ralstonia pickettii
H58Y	mutant, screening for thermostable enzyme	Ralstonia pickettii
Q12A	saturation mutagenesis at position Gln12, thermostability 13.3%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
Q12C	saturation mutagenesis at position Gln12, thermostability 9.3%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
Q12D	saturation mutagenesis at position Gln12, thermostability 8.9%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
Q12E	saturation mutagenesis at position Gln12, thermostability 9.5%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
Q12F	saturation mutagenesis at position Gln12, thermostability 14.3%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
Q12G	saturation mutagenesis at position Gln12, thermostability 20.5%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
Q12H	saturation mutagenesis at position Gln12, thermostability 12.8%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
Q12I	saturation mutagenesis at position Gln12, thermostability 14.5%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
Q12K	saturation mutagenesis at position Gln12, thermostability 11.0%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
Q12L	mutant, screening for thermostable enzyme	Ralstonia pickettii
Q12L	saturation mutagenesis at position Gln12, thermostability 47.2%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
Q12M	saturation mutagenesis at position Gln12, thermostability 9.2%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
Q12N	saturation mutagenesis at position Gln12, thermostability 15.5%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
Q12P	saturation mutagenesis at position Gln12, thermostability 12.0%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
Q12R	saturation mutagenesis at position Gln12, thermostability 9.2%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
Q12S	saturation mutagenesis at position Gln12, thermostability 12.0%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
Q12T	saturation mutagenesis at position Gln12, thermostability 14.8%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
Q12V	saturation mutagenesis at position Gln12, thermostability 20.3%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
Q12W	saturation mutagenesis at position Gln12, thermostability 16.5%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
Q12Y	saturation mutagenesis at position Gln12, thermostability 11.9%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
T262A	mutant, screening for thermostable enzyme	Ralstonia pickettii
T263A	saturation mutagenesis at position Thr263, thermostability 8.6%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
T263C	saturation mutagenesis at position Thr263, thermostability 13.2%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
T263D	saturation mutagenesis at position Thr263, thermostability 10.3%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
T263E	saturation mutagenesis at position Thr263, thermostability 12.5%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
T263F	saturation mutagenesis at position Thr263, thermostability 13.0%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
T263G	saturation mutagenesis at position Thr263, thermostability 15.7%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
T263H	saturation mutagenesis at position Thr263, thermostability 15.0%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
T263I	saturation mutagenesis at position Thr263, thermostability 14.7%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
T263K	saturation mutagenesis at position Thr263, thermostability 11.8%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
T263L	saturation mutagenesis at position Thr263, thermostability 10.4%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
T263M	saturation mutagenesis at position Thr263, thermostability 23.0%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
T263N	saturation mutagenesis at position Thr263, thermostability 14.7%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
T263P	saturation mutagenesis at position Thr263, thermostability 14.6%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
T263Q	saturation mutagenesis at position Thr263, thermostability 10.7%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
T263R	saturation mutagenesis at position Thr263, thermostability 9.6%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
T263S	mutant, screening for thermostable enzyme	Ralstonia pickettii
T263S	saturation mutagenesis at position Thr263, thermostability 25.0%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
T263V	saturation mutagenesis at position Thr263, thermostability 8.7%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
T263W	saturation mutagenesis at position Thr263, thermostability 9.5%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
T263Y	saturation mutagenesis at position Thr263, thermostability 12.0%, wild-type 12.3%, incubation at various temperatures, residual activity is calculated relative to the activity of the non-heat treated enzyme	Ralstonia pickettii
V237A	mutant, screening for thermostable enzyme	Ralstonia pickettii

Organism

Organism	UniProt	Comment	Textmining
Ralstonia pickettii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Ralstonia pickettii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-carbamoyl-D-p-hydroxyphenylglycine + H2O	activity assay	Ralstonia pickettii	D-p-hydroxyphenylglycine + NH3 + CO2	-	?

Synonyms

Synonyms	Comment	Organism
Dcase	-	Ralstonia pickettii
N-Carbamyl-D-amino acid amidohydrolase	-	Ralstonia pickettii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	activity assay	Ralstonia pickettii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	activity assay	Ralstonia pickettii