Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuSO4 | 10 mM: strong inhibition | Pseudomonas aeruginosa | |
EDTA | 10 mM: 30% inhibition | Pseudomonas aeruginosa | |
HgCl2 | 1 mM: strong inhibition | Pseudomonas aeruginosa | |
iodoacetate | 2 mM: strong inhibition, protected partially, about 30%, by preincubation, 15 min, with 20 mM 2-mercaptoethanol | Pseudomonas aeruginosa | |
additional information | no inhibition by urea, p-chloromercuribenzoate, NaF or KF | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.8 | - |
L-Theanine | partial purified enzyme | Pseudomonas aeruginosa | |
9.2 | - |
D-theanine | partial purified enzyme | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no activation by phosphate ions | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N5-ethyl-L-glutamine + H2O | Pseudomonas aeruginosa | - |
L-glutamate + ethylamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
36.83 | - |
- |
Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glutamine + H2O | - |
Pseudomonas aeruginosa | D-glutamate + NH3 | - |
? | |
L-glutamine + H2O | - |
Pseudomonas aeruginosa | L-glutamate + NH3 | - |
? | |
additional information | substrate specificity | Pseudomonas aeruginosa | ? | - |
? | |
additional information | no activity with L-asparagine, D-asparagine or acetamide | Pseudomonas aeruginosa | ? | - |
? | |
N5-ethyl-D-glutamine + H2O | D-theanine, probably by the same enzyme like L-theanine | Pseudomonas aeruginosa | D-glutamate + ethylamine | - |
? | |
N5-ethyl-L-glutamine + H2O | - |
Pseudomonas aeruginosa | L-glutamate + ethylamine | - |
? | |
theanine + hydroxylamine | transferase reaction | Pseudomonas aeruginosa | gamma-glutamylhydroxamic acid + ethylamine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
60 min, stable at over a wide range of pH | Pseudomonas aeruginosa |
55 | - |
above 55°C: rapid inactivation of enzyme | Pseudomonas aeruginosa |
60 | - |
15 min, rapid inactivation of enzyme | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pseudomonas aeruginosa |
8.5 | 9 | - |
Pseudomonas aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10.5 | about half of maximum activity at pH 5.5 and 10.5 | Pseudomonas aeruginosa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9.5 | quite stable between | Pseudomonas aeruginosa |