Cloned (Comment) | Organism |
---|---|
cDNA truncated at the N-terminus by 14 residues, overexpression in Escherichia coli BL21 | Arabidopsis sp. |
General Stability | Organism |
---|---|
dithiothreitol and glycerol stabilize | Arabidopsis sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-2-phenylpropionic acid | IC50: 0.115 mM | Zea mays | |
(RS)-2-(2,6-dinitrophenoxy)-propionate | most potent inhibitor of aryl propionates, IC50: 500 nM, completely reversible by passing the enzyme-inhibitor complex over a gel filtration column | Zea mays | |
(RS)-2-benzylpropionic acid | IC50: 0.006 mM | Zea mays | |
(S)-2-phenylpropionic acid | IC50: 0.0057 mM | Zea mays | |
1,10-phenanthroline | 1 mM, 96% inhibition, activity can be partially restored by Zn2+ | Zea mays | |
2,6-dipicolinic acid | 1 mM, 33% inhibition | Zea mays | |
2-methyl-N-carbamoyl-beta-alanine | competitive inhibitor of the reaction with N-carbamoyl-beta-alanine | Zea mays | |
3-Phenylpropionic acid | IC50: 0.0056 mM | Zea mays | |
8-hydroxyquinoline | 1 mM, 29% inhibition | Zea mays | |
Cu2+ | 0.2 mM, complete inhibition | Zea mays | |
cyclopropanecarboxylic acid | IC50: 0.008 mM | Zea mays | |
EDTA | 1 mM, 56% inhibition | Zea mays | |
iodoacetamide | 1 mM, complete inhibition, completely prevented by addition of substrate, the sensitive thiol group is located at the active site | Zea mays | |
isobutyric acid | IC50: 0.018 mM | Zea mays | |
additional information | not inhibited by hydroxyurea, acetohydroxamic acid, benzenesulfonamide, 4-carboxybenzenesulfonamide, 4-nitrobenzenesulfonamide, all up to 1 mM, substrate analogs, such as alpha-ureidopropionate, alpha-ureidoisobutyrate, alpha-ureido-n-butyrate, N-carbamoyl-Asp, or product beta-alanine, all up to 2 mM | Zea mays | |
n-butyric acid | IC50: 0.055 mM | Zea mays | |
p-chloromercuribenzenesulfonic acid | complete inhibition | Zea mays | |
propionic acid | IC50: 0.025 mM | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
2-methyl-N-carbamoyl-beta-alanine | pH 7, 25°C | Zea mays | |
0.011 | - |
N-Carbamoyl-beta-alanine | pH 7, 25°C | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | recombinant beta-UP expressed in Escherichia coli BL21 | Arabidopsis sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | Zn2+-dependent enzyme, required as a catalytic cofactor | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
10 * 43000, recombinant beta-UP, at pH 7, SDS-PAGE, 10 * 43723, recombinant beta-UP, at pH 7, mass spectrometry, 10 * 43733, sequence calculation | Arabidopsis sp. |
43723 | - |
10 * 43000, recombinant beta-UP, at pH 7, SDS-PAGE, 10 * 43723, recombinant beta-UP, at pH 7, mass spectrometry, 10 * 43733, sequence calculation | Arabidopsis sp. |
43733 | - |
10 * 43000, recombinant beta-UP, at pH 7, SDS-PAGE, 10 * 43723, recombinant beta-UP, at pH 7, mass spectrometry, 10 * 43733, sequence calculation | Arabidopsis sp. |
90000 | - |
gel filtration at pH 5.6, in the absence of glycerol | Arabidopsis sp. |
400000 | - |
above, native state of beta-UP, gel filtration at pH 7.3, in the presence of 5% v/v glycerol | Arabidopsis sp. |
440000 | - |
native state of beta-UP, gel filtration | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyl-N-carbamoyl-beta-alanine + H2O | Zea mays | final enzyme of the catabolic pathway of pyrimidine bases, substrate derives from thymine | 2-methyl-beta-alanine + CO2 + NH3 | - |
? | |
2-methyl-N-carbamoyl-beta-alanine + H2O | Arabidopsis sp. | final enzyme of the catabolic pathway of pyrimidine bases, substrate derives from thymine | 2-methyl-beta-alanine + CO2 + NH3 | - |
? | |
N-carbamoyl-beta-alanine + H2O | Zea mays | final enzyme of the catabolic pathway of pyrimidine bases, substrate derives from uracil | beta-alanine + CO2 + NH3 | - |
? | |
N-carbamoyl-beta-alanine + H2O | Arabidopsis sp. | final enzyme of the catabolic pathway of pyrimidine bases, substrate derives from uracil | beta-alanine + CO2 + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis sp. | - |
- |
- |
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Zea mays |
recombinant beta-UP | Arabidopsis sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
3-ureidopropanoate + H2O = beta-alanine + CO2 + NH3 | mechanism | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
shoot | of etiolated seedlings | Zea mays | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.03 | - |
recombinant beta-UP | Arabidopsis sp. |
Storage Stability | Organism |
---|---|
-70°C, partially purified beta-UP, in presence of 10% w/v glycerol, several months, stable | Zea mays |
4°C, partially purified beta-UP, in presence of Mg2+ and dithiothreitol, 7 days, about 10% loss of activity | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyl-N-carbamoyl-beta-alanine + H2O | - |
Arabidopsis sp. | 2-methyl-beta-alanine + CO2 + NH3 | - |
? | |
2-methyl-N-carbamoyl-beta-alanine + H2O | final enzyme of the catabolic pathway of pyrimidine bases, substrate derives from thymine | Zea mays | 2-methyl-beta-alanine + CO2 + NH3 | - |
? | |
2-methyl-N-carbamoyl-beta-alanine + H2O | final enzyme of the catabolic pathway of pyrimidine bases, substrate derives from thymine | Arabidopsis sp. | 2-methyl-beta-alanine + CO2 + NH3 | - |
? | |
2-methyl-N-carbamoyl-beta-alanine + H2O | only one enantiomer of the racemic substrate is hydrolyzed | Zea mays | 2-methyl-beta-alanine + CO2 + NH3 | - |
? | |
N-carbamoyl-beta-alanine + H2O | - |
Zea mays | beta-alanine + CO2 + NH3 | - |
? | |
N-carbamoyl-beta-alanine + H2O | - |
Arabidopsis sp. | beta-alanine + CO2 + NH3 | - |
? | |
N-carbamoyl-beta-alanine + H2O | final enzyme of the catabolic pathway of pyrimidine bases, substrate derives from uracil | Zea mays | beta-alanine + CO2 + NH3 | - |
? | |
N-carbamoyl-beta-alanine + H2O | final enzyme of the catabolic pathway of pyrimidine bases, substrate derives from uracil | Arabidopsis sp. | beta-alanine + CO2 + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | - |
Zea mays |
decamer | 10 * 43000, recombinant beta-UP, at pH 7, SDS-PAGE, 10 * 43723, recombinant beta-UP, at pH 7, mass spectrometry, 10 * 43733, sequence calculation | Arabidopsis sp. |
dimer | recombinant beta-UP is dimeric at pH 5.6, in the absence of glycerol | Arabidopsis sp. |
Synonyms | Comment | Organism |
---|---|---|
beta-Ala synthase | - |
Zea mays |
beta-UP | - |
Zea mays |
beta-UP | - |
Arabidopsis sp. |
N-carbamoyl-beta-Ala amidohydrolase | - |
Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7.2 | broad optimum at mildly acidic pH values | Zea mays |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
activity declines at more alkaline pH values | Zea mays |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Arabidopsis sp. | recombinant beta-UP, isoelectric focusing | - |
6 |
Arabidopsis sp. | recombinant beta-UP, sequence calculation | - |
7.1 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0005 | - |
most potent inhibitor of aryl propionates, IC50: 500 nM, completely reversible by passing the enzyme-inhibitor complex over a gel filtration column | Zea mays | (RS)-2-(2,6-dinitrophenoxy)-propionate | |
0.0056 | - |
IC50: 0.0056 mM | Zea mays | 3-Phenylpropionic acid | |
0.0057 | - |
IC50: 0.0057 mM | Zea mays | (S)-2-phenylpropionic acid | |
0.006 | - |
IC50: 0.006 mM | Zea mays | (RS)-2-benzylpropionic acid | |
0.008 | - |
IC50: 0.008 mM | Zea mays | cyclopropanecarboxylic acid | |
0.018 | - |
IC50: 0.018 mM | Zea mays | isobutyric acid | |
0.025 | - |
IC50: 0.025 mM | Zea mays | propionic acid | |
0.055 | - |
IC50: 0.055 mM | Zea mays | n-butyric acid | |
0.115 | - |
IC50: 0.115 mM | Zea mays | (R)-2-phenylpropionic acid |