Application | Comment | Organism |
---|---|---|
synthesis | enzymatic method of L-tryptophan production. The enzyme is useful for the manufacturing process because it is not inhibited by high levels of product | Cutaneotrichosporon cutaneum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-dipyridyl | - |
Cutaneotrichosporon cutaneum | |
additional information | no inhibition by high levels of the product L-tryptophan | Cutaneotrichosporon cutaneum | |
NEM | - |
Cutaneotrichosporon cutaneum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Cutaneotrichosporon cutaneum | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 25% of the activation with Mn2+ | Cutaneotrichosporon cutaneum | |
Mn2+ | 2.5 mM required for full activity | Cutaneotrichosporon cutaneum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
4 * 68000, SDS-PAGE | Cutaneotrichosporon cutaneum |
270000 | - |
gel filtration | Cutaneotrichosporon cutaneum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cutaneotrichosporon cutaneum | - |
IFO 0173 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alaninamide + H2O | hydrolyzed 14% as rapidly as L-tryptophanamide | Cutaneotrichosporon cutaneum | L-alanine + NH3 | - |
? | |
L-asparagine + H2O | hydrolyzed 22% as rapidly as L-tryptophanamide | Cutaneotrichosporon cutaneum | L-aspartate + NH3 | - |
? | |
L-citrulline + H2O | hydrolyzed 22% as rapidly as L-tryptophanamide | Cutaneotrichosporon cutaneum | ? | - |
? | |
L-glutamine + H2O | hydrolyzed 18% as rapidly as L-tryptophanamide | Cutaneotrichosporon cutaneum | L-glutamate + NH3 | - |
? | |
L-leucinamide + H2O | hydrolyzed 26% as rapidly as L-tryptophanamide | Cutaneotrichosporon cutaneum | L-leucine + NH3 | - |
? | |
L-methioninamide + H2O | hydrolyzed 14% as rapidly as L-tryptophanamide | Cutaneotrichosporon cutaneum | L-methionine + NH3 | - |
? | |
L-phenylalaninamide + H2O | hydrolyzed 47% as rapidly as L-tryptophanamide | Cutaneotrichosporon cutaneum | L-phenylalanine + NH3 | - |
? | |
L-serinamide + H2O | hydrolyzed 7% as rapidly as L-tryptophanamide | Cutaneotrichosporon cutaneum | L-serine + NH3 | - |
? | |
L-tryptophanamide + H2O | - |
Cutaneotrichosporon cutaneum | L-tryptophan + NH3 | - |
? | |
L-tyrosinamide + H2O | hydrolyzed 22% as rapidly as L-tryptophanamide | Cutaneotrichosporon cutaneum | L-tyrosine + NH3 | - |
? | |
L-valinamide + H2O | hydrolyzed 15% as rapidly as L-tryptophanamide | Cutaneotrichosporon cutaneum | L-valine + NH3 | - |
? | |
additional information | high affinity towards peptides having a L-Trp residue at the N-terminal moiety | Cutaneotrichosporon cutaneum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 68000, SDS-PAGE | Cutaneotrichosporon cutaneum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | - |
Cutaneotrichosporon cutaneum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
10 min, stable | Cutaneotrichosporon cutaneum |
60 | - |
rapid inactivation | Cutaneotrichosporon cutaneum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 9.5 | - |
Cutaneotrichosporon cutaneum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | room temperature, 20 h, no appreciable loss of activity | Cutaneotrichosporon cutaneum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cutaneotrichosporon cutaneum | isoelectrofocusing | - |
4.7 |