Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Flavobacterium sp. |
Protein Variants | Comment | Organism |
---|---|---|
D181E | mutant with increased Km and decreased activity | Flavobacterium sp. |
D181H | - |
Flavobacterium sp. |
D181K | - |
Flavobacterium sp. |
D181N | mutant with increased Km and decreased activity | Flavobacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
80 | - |
N-(6-aminohexanoyl)-6-aminohexanoate | mutation D181N | Flavobacterium sp. | |
220 | - |
N-(6-aminohexanoyl)-6-aminohexanoate | mutation D181E | Flavobacterium sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Flavobacterium sp. | 5622 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flavobacterium sp. | - |
- |
- |
Flavobacterium sp. KI72 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques, recombinant enzyme | Flavobacterium sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | nylB gene, enzyme EII | Flavobacterium sp. | - |
cell culture | nylB' gene, enzyme EII' | Flavobacterium sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-(6-aminohexanoyl)-6-aminohexanoate + H2O | mutant protein D181N 20fold less active, mutant D181E 200fold less active | Flavobacterium sp. | 6-aminohexanoate + 6-aminohexanoate | - |
? | |
N-(6-aminohexanoyl)-6-aminohexanoate + H2O | mutant protein D181N 20fold less active, mutant D181E 200fold less active | Flavobacterium sp. KI72 | 6-aminohexanoate + 6-aminohexanoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EII | - |
Flavobacterium sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
N-(6-aminohexanoyl)-6-aminohexanoate | single mutation D181E | Flavobacterium sp. | |
2.7 | - |
N-(6-aminohexanoyl)-6-aminohexanoate | single mutation D181N | Flavobacterium sp. | |
8.9 | - |
N-(6-aminohexanoyl)-6-aminohexanoate | EII without mutation | Flavobacterium sp. |