Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Burkholderia sp. | |
Al3+ | - |
Burkholderia sp. | |
As3+ | - |
Burkholderia sp. | |
Cd2+ | - |
Burkholderia sp. | |
Co2+ | - |
Burkholderia sp. | |
Cr2+ | - |
Burkholderia sp. | |
Cs+ | - |
Burkholderia sp. | |
Cu2+ | - |
Burkholderia sp. | |
Fe2+ | - |
Burkholderia sp. | |
Hg2+ | - |
Burkholderia sp. | |
Li+ | - |
Burkholderia sp. | |
Mg2+ | - |
Burkholderia sp. | |
Mn2+ | - |
Burkholderia sp. | |
additional information | no inhibition by 2-mercaptoethanol and dithiotreitol; the metal ions tested show between 30% and 40% inhibition at 1 mM | Burkholderia sp. | |
Ni2+ | - |
Burkholderia sp. | |
Pb2+ | - |
Burkholderia sp. | |
Se2+ | - |
Burkholderia sp. | |
WO42- | - |
Burkholderia sp. | |
Zn2+ | - |
Burkholderia sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
acetamide | pH 7.5, 30°C | Burkholderia sp. | |
0.27 | - |
acetamide | pH 8.0, 40°C | Burkholderia sp. | |
1.21 | - |
2-chloroacetamide | pH 7.5, 30°C | Burkholderia sp. | |
1.21 | - |
2-chloroacetamide | pH 8.0, 40°C | Burkholderia sp. | |
1.88 | - |
Urea | pH 7.5, 30°C | Burkholderia sp. | |
1.88 | - |
Urea | pH 8.0, 40°C | Burkholderia sp. | |
2.39 | - |
Acrylamide | pH 7.5, 30°C | Burkholderia sp. | |
2.39 | - |
Acrylamide | pH 8.0, 40°C | Burkholderia sp. | |
4.29 | - |
Propionamide | pH 7.5, 30°C | Burkholderia sp. | |
4.29 | - |
Propionamide | pH 8.0, 40°C | Burkholderia sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
4 * 47000, SDS-PAGE | Burkholderia sp. |
186000 | - |
gel filtration | Burkholderia sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia sp. | - |
an acrylamide-degrading bacterium isolated from the grounds of a palm estate in Serdang, Selangor | - |
Burkholderia sp. | - |
previously isolated from the grounds of a palm estate in Serdang, Selangor | - |
Burkholderia sp. DR.Y27 | - |
an acrylamide-degrading bacterium isolated from the grounds of a palm estate in Serdang, Selangor | - |
Burkholderia sp. DR.Y27 | - |
previously isolated from the grounds of a palm estate in Serdang, Selangor | - |
Purification (Comment) | Organism |
---|---|
native enzyme 11.15fold to homogeneity by two steps of anion exchange chromatography, followed by gel filtration | Burkholderia sp. |
native enzyme 11.2fold by anion exchange chromatography, and gel filtration | Burkholderia sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10 | - |
purified enzyme, pH 7.5, 30°C | Burkholderia sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chloroacetamide + H2O | - |
Burkholderia sp. | 2-chloroacetate + NH3 | - |
? | |
acetamide + H2O | - |
Burkholderia sp. | acetate + NH3 | - |
? | |
acetamide + H2O | best substrate | Burkholderia sp. | acetate + NH3 | - |
? | |
acetamide + H2O | - |
Burkholderia sp. DR.Y27 | acetate + NH3 | - |
? | |
acetamide + H2O | best substrate | Burkholderia sp. DR.Y27 | acetate + NH3 | - |
? | |
acrylamide + H2O | - |
Burkholderia sp. | acrylate + NH3 | - |
? | |
acrylamide + H2O | best substrate | Burkholderia sp. | acrylate + NH3 | - |
? | |
acrylamide + H2O | - |
Burkholderia sp. DR.Y27 | acrylate + NH3 | - |
? | |
additional information | no activity with methacrylamide and nicotinamide | Burkholderia sp. | ? | - |
? | |
additional information | methacrylamide and nicotinamide are no substrates | Burkholderia sp. | ? | - |
? | |
additional information | no activity with methacrylamide and nicotinamide | Burkholderia sp. DR.Y27 | ? | - |
? | |
additional information | methacrylamide and nicotinamide are no substrates | Burkholderia sp. DR.Y27 | ? | - |
? | |
propionamide + H2O | - |
Burkholderia sp. | propionate + NH3 | - |
? | |
propionamide + H2O | - |
Burkholderia sp. | propanoate + NH3 | - |
? | |
urea + H2O | reaction of EC 3.5.1.5 | Burkholderia sp. | CO2 + 2 NH3 | - |
? | |
urea + H2O | - |
Burkholderia sp. | carbamic acid + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 47000, SDS-PAGE | Burkholderia sp. |
tetramer | 4 * 47000, SDS-PAGE | Burkholderia sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Burkholderia sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 60 | activity range, sharp drop in activity above 50°C | Burkholderia sp. |
35 | 60 | activity range, activity decreases sharply at higher temperatures | Burkholderia sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Burkholderia sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.7 | 10.5 | activity range, 61% of maximal activity at pH 5.7, 30% at pH 10.5 | Burkholderia sp. |
7.5 | 8 | activity range, at pH 5.7 and pH 9.5, the enzyme loses 39% and 33% of its activity respectively, and at pH 10.5, the loss in enzyme activity is at 70% | Burkholderia sp. |