Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.4 extracted from

  • Mayaux, J.F.; Cerbelaud, E.; Soubrier, F.; Yeh, P.; Blanche, F.; Petre, D.
    Purification, cloning and primary structure of a new enantiomer-selective amidase from a Rhodococcus Strain: structural evidence for a conserved genetic coupling with nitrile hydratase (1991), J. Bacteriol., 173, 6694-6704.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Brevibacterium lactofermentum, but not in Escherichia coli by using a pSR1-derived shuttle vector Rhodococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48554
-
 2 * 48554 Rhodococcus sp.
118000
-
 gel filtration Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Brevibacterium sp.
-
-
-
Brevibacterium sp. R312
-
-
-
Rhodococcus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
prepared of a crude extract by ultrasonic treatment, purification and concentration with ion-exchange chromatography, hydrophobic chromatography, hydroxyapatite chromatography Rhodococcus sp.

Reaction

Reaction Comment Organism Reaction ID
a monocarboxylic acid amide + H2O = a monocarboxylate + NH3 stereospecific hydrolysis of 2-arylpropionamides Rhodococcus sp.
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.5
-
 specific activity of S(+)-2-phenylpropionic acid Rhodococcus sp.

Storage Stability

Storage Stability Organism
-20°C 0.35 M potassium phosphate with 0.5 mM DTT, 10 micromol CaCl2 and 15% glycerol, several weeks without loss of activity Rhodococcus sp.

Subunits

Subunits Comment Organism
dimer
-
 Brevibacterium sp.
dimer 2 * 48554 Rhodococcus sp.