KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.88 | - |
N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine | - |
Spodoptera frugiperda |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17000 | - |
alpha 1, 1 * 28000 + beta 1, 1 * 17000, SDS-PAGE | Spodoptera frugiperda |
17000 | - |
alpha2,beta2, 2 * 28000 + 2 * 17000, SDS-PAGE | Spodoptera frugiperda |
28000 | - |
alpha 1, 1 * 28000 + beta 1, 1 * 17000, SDS-PAGE | Spodoptera frugiperda |
28000 | - |
alpha2,beta2, 2 * 28000 + 2 * 17000, SDS-PAGE | Spodoptera frugiperda |
47000 | - |
gel filtration | Spodoptera frugiperda |
101000 | - |
gel filtration | Spodoptera frugiperda |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | Spodoptera frugiperda | - |
L-aspartate + NH3 | - |
? | |
N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O | Bos taurus | - |
2-acetamido-1-amino-1,2-dideoxy-beta-D-glucose + L-aspartate | - |
? | |
N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O | Spodoptera frugiperda | - |
2-acetamido-1-amino-1,2-dideoxy-beta-D-glucose + L-aspartate | - |
? | |
N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O | Rattus sp. | - |
2-acetamido-1-amino-1,2-dideoxy-beta-D-glucose + L-aspartate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Rattus sp. | - |
- |
- |
Spodoptera frugiperda | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Spodoptera frugiperda |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | - |
Bos taurus | - |
placenta | - |
Rattus sp. | - |
SF-9 cell | - |
Spodoptera frugiperda | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.1 | - |
- |
Spodoptera frugiperda |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | - |
Spodoptera frugiperda | L-aspartate + NH3 | - |
? | |
N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O | - |
Bos taurus | 2-acetamido-1-amino-1,2-dideoxy-beta-D-glucose + L-aspartate | - |
? | |
N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O | - |
Spodoptera frugiperda | 2-acetamido-1-amino-1,2-dideoxy-beta-D-glucose + L-aspartate | - |
? | |
N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O | - |
Rattus sp. | 2-acetamido-1-amino-1,2-dideoxy-beta-D-glucose + L-aspartate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Bos taurus |
dimer | - |
Rattus sp. |
dimer | alpha 1, 1 * 28000 + beta 1, 1 * 17000, SDS-PAGE | Spodoptera frugiperda |
tetramer | alpha2,beta2, 2 * 28000 + 2 * 17000, SDS-PAGE | Spodoptera frugiperda |
Synonyms | Comment | Organism |
---|---|---|
glycosylasparaginase | - |
Spodoptera frugiperda |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 70 | denaturation | Spodoptera frugiperda |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10.5 | - |
Spodoptera frugiperda |