Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.23 extracted from

  • Chen, L.Y.; Shi, D.Q.; Zhang, W.J.; Tang, Z.S.; Liu, J.; Yang, W.C.
    The Arabidopsis alkaline ceramidase TOD1 is a key turgor pressure regulator in plant cells (2015), Nat. Commun., 6, 6030.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.23

Cloned(Commentary)

Cloned (Comment) Organism
gene tod1, DNA and amino acid sequence determination and analysis, genetic structure, phylogenetic tree Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information construction of T-DNA insertion mutants harboring the mutation in exn tod1-1 or tod1-2 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
 Arabidopsis thaliana 5794
-
microsome
-
 Arabidopsis thaliana
-
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q6DBD7 gene tod1
-
Arabidopsis thaliana Landsberg erecta Q6DBD7 gene tod1
-

Source Tissue

Source Tissue Comment Organism Textmining
guard cell from siliques Arabidopsis thaliana
-
additional information enzyme expression analysis Arabidopsis thaliana
-
pollen tubes Arabidopsis thaliana
-
silique
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
C12-NBD-Cer + H2O
-
 Arabidopsis thaliana NBD-dodecanoic acid + sphingosine
-
 ?
C12-NBD-Cer + H2O
-
 Arabidopsis thaliana Landsberg erecta NBD-dodecanoic acid + sphingosine
-
 ?

Subunits

Subunits Comment Organism
More the enzyme has a transmembrane domain (residues 20-39), and a DUF616 domain (residues 107-409) Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
alkaline ceramidase
-
 Arabidopsis thaliana
TOD1
-
 Arabidopsis thaliana
turgor regulation defect 1
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction enzyme-deficient tod1 mutant pollen tubes have higher turgor than wild-type and show growth retardation both in pistils and in agarose medium. In addition, tod1 mutant guard cells are insensitive to abscisic acid-induced stomatal closure, whereas sphingosine-1-phosphate, a putative downstream component of abscisic acid signalling and product of alkaline ceramidases, promotes closure in both wild-type and tod1, TOD1 is sufficient to rescue pollen tube growth defect. Pollen tube growth defects of tod1-2 are suppressed by the gaut13 mutation, a single-nucleotide substitution in the galacturonosyltransferase13 gene. Phenotypes, overview Arabidopsis thaliana
physiological function alkaline ceramidase TOD1 is preferentially expressed in pollen tubes and in silique guard cells, where it is required for turgor pressure regulation, it is a key turgor pressure regulator in plant cells, molecular mechanism. Turgor pressure plays pivotal roles in the growth and movement of walled cells Arabidopsis thaliana