Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Camelus dromedarius | |
Cu2+ | - |
Camelus dromedarius | |
cysteine | - |
Camelus dromedarius | |
GSH | - |
Camelus dromedarius | |
GSSG | - |
Camelus dromedarius | |
N-acetylcysteine | - |
Camelus dromedarius | |
phosphatidic acid | complete inhibition | Camelus dromedarius | |
phosphatidylcholine | complete inhibition | Camelus dromedarius | |
phosphatidylserine | complete inhibition | Camelus dromedarius | |
TMP | - |
Camelus dromedarius | |
UMP | - |
Camelus dromedarius | |
Zn2+ | - |
Camelus dromedarius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Camelus dromedarius | |
0.0498 | - |
C12-NBD-Cer | pH 7.5, 37°C | Camelus dromedarius |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound | Camelus dromedarius | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Camelus dromedarius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
x * 100000, about, glycosylated enzyme, SDS-PAGE, x * 80000, about, deglycosylated enzyme, SDS-PAGE | Camelus dromedarius |
100000 | - |
x * 100000, about, glycosylated enzyme, SDS-PAGE, x * 80000, about, deglycosylated enzyme, SDS-PAGE | Camelus dromedarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Camelus dromedarius | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | deeglycosylation by glycosidase F treatment | Camelus dromedarius |
Purification (Comment) | Organism |
---|---|
native enzyme 2147.1fold from brain to homogeneity by anion exchange and hydrophobic interaction chromtography, gel filtration, and another step of anion exchange chromatography | Camelus dromedarius |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Camelus dromedarius | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
230.6 | - |
purified enzyme, pH 7.5, 37°C | Camelus dromedarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
C12-NBD-Cer + H2O | the enzyme catalyzes also the reverse reaction of ceramide synthesis | Camelus dromedarius | NBD-dodecanoic acid + D-erythro-sphingosine | - |
r | |
N-hexanoyl-D-erythro-sphingosine + H2O | - |
Camelus dromedarius | hexanoate + D-erythro-sphingosine | - |
? | |
N-lauroyl-D-erythro-sphingosine + H2O | the enzyme catalyzes also the reverse reaction of ceramide synthesis | Camelus dromedarius | laurate + D-erythro-sphingosine | - |
r | |
N-lignoceroyl-D-erythro-sphingosine + H2O | low activity | Camelus dromedarius | lignocerate + D-erythro-sphingosine | - |
? | |
N-myristoyl-D-erythro-sphingosine + H2O | best substrate | Camelus dromedarius | myristate + D-erythro-sphingosine | - |
? | |
N-palmitoyl-D-erythro-sphingosine + H2O | - |
Camelus dromedarius | palmitate + D-erythro-sphingosine | - |
? | |
N-stearoyl-D-erythro-sphingosine + H2O | - |
Camelus dromedarius | stearate + D-erythro-sphingosine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 100000, about, glycosylated enzyme, SDS-PAGE, x * 80000, about, deglycosylated enzyme, SDS-PAGE | Camelus dromedarius |
Synonyms | Comment | Organism |
---|---|---|
CBCDase | - |
Camelus dromedarius |
nCDase | - |
Camelus dromedarius |
neutral ceramidase | - |
Camelus dromedarius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Camelus dromedarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Camelus dromedarius |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | activity range, high activity at pH 6.0-8.0, profile overview | Camelus dromedarius |