Cloned (Comment) | Organism |
---|---|
recombinant expression in insect cells | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, overview | Homo sapiens | |
0.036 | - |
N-[(2S,3R,4E)-13-[[9-(ethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]-1,3-dihydroxytridec-4-en-2-yl]hexadecanamide | pH 7.0, 37°C, recombinant enzyme | Homo sapiens | |
0.082 | - |
N-[(2S,3R,4E)-13-[[9-(ethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]-1,3-dihydroxytridec-4-en-2-yl]hexadecanamide | pH 4.5, 37°C, recombinant enzyme | Homo sapiens | |
0.085 | - |
N-[(2S,3R,4E)-1,3-dihydroxynonadec-4-en-2-yl]-12-[[9-(ethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]dodecanamide | pH 7.0, 37°C, recombinant enzyme | Homo sapiens | |
0.087 | - |
(2R,3Z)-2-([(2E)-1-hydroxy-12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodec-2-en-1-yl]amino)heptadec-3-ene-1,3-diol | pH 4.5, 37°C, recombinant enzyme | Homo sapiens | |
0.11 | - |
N-[(2S,3R,4E)-1,3-dihydroxynonadec-4-en-2-yl]-12-[[9-(ethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]dodecanamide | pH 4.5, 37°C, recombinant enzyme | Homo sapiens | |
0.19 | - |
(2R,3Z)-2-([(2E)-1-hydroxy-12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodec-2-en-1-yl]amino)heptadec-3-ene-1,3-diol | pH 7.0, 37°C, recombinant enzyme | Homo sapiens | |
0.193 | - |
N-[(2S,3R,4E)-1,3-dihydroxy-14-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]tetradec-4-en-2-yl]hexadecanamide | pH 7.0, 37°C, recombinant enzyme | Homo sapiens | |
0.204 | - |
N-[(2S,3R,4E)-1,3-dihydroxy-14-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]tetradec-4-en-2-yl]hexadecanamide | pH 4.5, 37°C, recombinant enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q13510 | - |
- |
Homo sapiens | Q9NR71 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from insect cells | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3Z)-2-([(2E)-1-hydroxy-12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodec-2-en-1-yl]amino)heptadec-3-ene-1,3-diol + H2O | 9.9% hydrolysis | Homo sapiens | ? | - |
ir | |
additional information | design and synthesis of a set of fluorescently labeled ceramides as substrates for acid and neutral ceramidases, overview. Although both the dyes, (acyl-(4-nitro-2,1,3-benzoxadiazol-7-yl)aminoethyl)-ceramide and acyl-Nile red-ceramide do not have any noticeable preference for the substitution at acyl or sphingosine part in ceramide towards hydrolysis by acid ceramidase, the ceramides with acyl-substituted NBD and Sph-substituted NR dyes have been found to be a better substrate for neutral ceramidase | Homo sapiens | ? | - |
? | |
N-[(2S,3R,4E)-1,3-dihydroxy-14-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]tetradec-4-en-2-yl]hexadecanamide + H2O | 5.6% hydrolysis | Homo sapiens | ? | - |
ir | |
N-[(2S,3R,4E)-1,3-dihydroxy-14-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]tetradec-4-en-2-yl]hexadecanamide + H2O | 6.2% hydrolysis | Homo sapiens | ? | - |
ir | |
N-[(2S,3R,4E)-1,3-dihydroxynonadec-4-en-2-yl]-12-[[9-(ethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]dodecanamide + H2O | 6.1% hydrolysis | Homo sapiens | ? | - |
ir | |
N-[(2S,3R,4E)-1,3-dihydroxynonadec-4-en-2-yl]-12-[[9-(ethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]dodecanamide + H2O | 63.3% hydrolysis | Homo sapiens | ? | - |
ir | |
N-[(2S,3R,4E)-13-[[9-(ethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]-1,3-dihydroxytridec-4-en-2-yl]hexadecanamide + H2O | 3.9% hydrolysis | Homo sapiens | ? | - |
ir | |
N-[(2S,3R,4E)-13-[[9-(ethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]-1,3-dihydroxytridec-4-en-2-yl]hexadecanamide + H2O | 6.3% hydrolysis | Homo sapiens | ? | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
aCDase | - |
Homo sapiens |
acid ceramidase | - |
Homo sapiens |
nCDase | - |
Homo sapiens |
neutral ceramidase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
assay at | Homo sapiens |
7 | - |
assay at | Homo sapiens |