Cloned (Comment) | Organism |
---|---|
heterologously expressed in Lactobacillus plantarum KLB213 which exhibits a high salt-tolerance and Lactobacillus paracasei KLB58 which produces high amount of exopolysaccharide | Limosilactobacillus reuteri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.5 | - |
L-glutamine | pH 7.5, 37°C | Limosilactobacillus reuteri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | enzyme is salt-tolerant as the relative activity displays 110% in the condition of 5% NaCl and remains over 50% in the presence of 20% NaCl | Limosilactobacillus reuteri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Limosilactobacillus reuteri | - |
KCTC3594 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the recombinant enzymes from Lactobacillus plantarum and Lactobacillus paracasei show increased enzyme activity. The wild-type organisms do not amplify the glutaminase gene. The recombinant Lactobacillus plantarum displays ca. 4fold higher activity than its wild-type and 1.5fold higher activity the wild-type Lactobacillus reuteri. The recombinant Lactobacillus paracasei enzyme increases to half of the activity of Lactobacillus reuteri. The wild-type Lactobacillus paracasei shows no glutaminase activity | Limosilactobacillus reuteri |
0.516 | - |
pH 7.5, 40°C, Lactobacillus reuteri shows the highest activity compared to wild-type Lactobacillus plantarum and wild-type Lactobacillus paracasei which are transformed with the glutaminase gene Lactobacillus reuteri in this study. The wild-type Lactobacillus paracasei shows no glutaminase activity | Limosilactobacillus reuteri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + H2O | specifc for L-glutamine | Limosilactobacillus reuteri | L-glutamate + NH3 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Limosilactobacillus reuteri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
78% activity remains after incubation at 60°C for 30 min | Limosilactobacillus reuteri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Limosilactobacillus reuteri |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 11 | stable at | Limosilactobacillus reuteri |