Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
G261A | 54,7% wild-type activity, Km: 23.7 mM | Escherichia coli |
Q162A | wild-type level of activity | Escherichia coli |
S260A | 16.5% of wild-type activity | Escherichia coli |
S66A | very low activity | Escherichia coli |
Y29A | 32.5% of wild-type activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-diazo-5-oxo-L-norleucine | - |
Bacillus subtilis | |
6-diazo-5-oxo-L-norleucine | - |
Escherichia coli | |
ampicillin | - |
Escherichia coli | |
Mg2+ | - |
Bacillus subtilis | |
Mg2+ | - |
Escherichia coli | |
Mn2+ | - |
Bacillus subtilis | |
Mn2+ | - |
Escherichia coli | |
penicillin | - |
Escherichia coli | |
PO43- | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.3 | - |
L-glutamine | - |
Escherichia coli | |
7.6 | - |
L-glutamine | - |
Bacillus subtilis | |
8.5 | - |
L-glutamine | mutant Q162A | Escherichia coli | |
23.7 | - |
L-glutamine | mutant G261A | Escherichia coli | |
27.6 | - |
L-glutamine | - |
Bacillus subtilis | |
30.6 | - |
L-glutamine | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32900 | - |
4 * 32900, SDS-PAGE | Escherichia coli |
33500 | - |
2 * 33500, SDS-PAGE | Escherichia coli |
34000 | - |
2 * 34000, SDS-PAGE | Bacillus subtilis |
36200 | - |
4 * 36200, SDS-PAGE, gelfiltration | Bacillus subtilis |
72600 | - |
gel filtration | Bacillus subtilis |
74900 | - |
gel filtration | Escherichia coli |
138100 | - |
gel filtration | Bacillus subtilis |
148800 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O07637 | ATCC 23857D-5 | - |
Bacillus subtilis | O31465 | ATCC 23857D-5 | - |
Bacillus subtilis 168 | O07637 | ATCC 23857D-5 | - |
Bacillus subtilis 168 | O31465 | ATCC 23857D-5 | - |
Escherichia coli | P0A6W0 | - |
- |
Escherichia coli | P77454 | - |
- |
Escherichia coli W3110 / ATCC 27325 | P0A6W0 | - |
- |
Escherichia coli W3110 / ATCC 27325 | P77454 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Bacillus subtilis |
Storage Stability | Organism |
---|---|
stable at -196°C (unstable at -80°C) | Escherichia coli |
stable at -80?C | Bacillus subtilis |
stable at -80°C | Bacillus subtilis |
stable at -80°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate-p-nitroanilide + H2O | - |
Escherichia coli | L-glutamate + 4-nitroaniline | - |
? | |
L-glutamate-p-nitroanilide + H2O | - |
Bacillus subtilis | L-glutamate + 4-nitroaniline | - |
? | |
L-glutamate-p-nitroanilide + H2O | - |
Bacillus subtilis 168 | L-glutamate + 4-nitroaniline | - |
? | |
L-glutamate-p-nitroanilide + H2O | - |
Escherichia coli W3110 / ATCC 27325 | L-glutamate + 4-nitroaniline | - |
? | |
L-glutamine + H2O | - |
Escherichia coli | L-glutamic acid + NH3 | - |
? | |
L-glutamine + H2O | - |
Bacillus subtilis | L-glutamic acid + NH3 | - |
? | |
L-glutamine + H2O | - |
Bacillus subtilis 168 | L-glutamic acid + NH3 | - |
? | |
L-glutamine + H2O | - |
Escherichia coli W3110 / ATCC 27325 | L-glutamic acid + NH3 | - |
? | |
nitrocefin + H2O | - |
Escherichia coli | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
nitrocefin + H2O | - |
Escherichia coli W3110 / ATCC 27325 | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 34000, SDS-PAGE | Bacillus subtilis |
homodimer | 2 * 33500, SDS-PAGE | Escherichia coli |
homotetramer | 4 * 32900, SDS-PAGE | Escherichia coli |
homotetramer | 4 * 36200, SDS-PAGE, gelfiltration | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
glutaminase | - |
Escherichia coli |
glutaminase | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
38.6 | - |
L-glutamine | - |
Bacillus subtilis | |
49.9 | - |
L-glutamine | mutant Q161A | Escherichia coli | |
67.7 | - |
L-glutamine | - |
Bacillus subtilis | |
79.9 | - |
L-glutamine | mutant Q162A | Escherichia coli | |
91.4 | - |
L-glutamine | - |
Escherichia coli | |
101 | - |
L-glutamine | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Escherichia coli |
7.5 | 8 | - |
Bacillus subtilis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
3.5 | - |
ampicillin | competitive inhibitor | Escherichia coli | |
6.4 | - |
penicillin | - |
Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.1 | - |
- |
Escherichia coli | Mn2+ | |
0.2 | - |
- |
Escherichia coli | Mg2+ | |
0.7 | 1.3 | - |
Bacillus subtilis | Mn2+ | |
0.7 | 1.3 | - |
Escherichia coli | Mn2+ | |
1.7 | 5.5 | - |
Bacillus subtilis | Mg2+ | |
1.7 | 5.5 | - |
Escherichia coli | Mg2+ | |
17.2 | 17.4 | - |
Escherichia coli | PO43- |