BRENDA - Enzyme Database
show all sequences of 3.5.1.16

Evolution of arginine biosynthesis in the bacterial domain: Novel gene-enzyme relationships from psychrophilic Moritella strains (Vibrionaceae) and evolutionary significance of N-alpha-acetyl ornithinase

Xu, Y.; Liang, Z.; Legrain, C.; Rueger, H.J.; Glansdorff, N.; J. Bacteriol. 182, 1609-1615 (2000)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
Escherichia coli transformed with pTrc99A + pC-28 containing argE, lambdaZAP library from 2674
Escherichia coli
Escherichia coli transformed with pTrc99A + pC-28 containing argE, lambdaZAP library from 2674
Moritella profunda
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
N2-acety-L-ornithine + H2O
Moritella abyssi
-
L-ornithine + acetate
-
-
?
N2-acety-L-ornithine + H2O
Escherichia coli
step in arginine biosynthesis
L-ornithine + acetate
-
-
?
N2-acety-L-ornithine + H2O
Moritella profunda
step in arginine biosynthesis
L-ornithine + acetate
-
-
?
N2-acety-L-ornithine + H2O
Escherichia coli 160-37
step in arginine biosynthesis
L-ornithine + acetate
-
-
?
N2-acety-L-ornithine + H2O
Escherichia coli ATCC 9637
step in arginine biosynthesis
L-ornithine + acetate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
-
K-12
-
Escherichia coli
-
160-37
-
Escherichia coli
-
ATCC9637
-
Escherichia coli 160-37
-
160-37
-
Escherichia coli ATCC 9637
-
ATCC9637
-
Moritella abyssi
-
psychrophilic Vibrionaceae
-
Moritella profunda
-
psychrophilic Vibrionaceae
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
N2-acety-L-ornithine + H2O
-
171963
Moritella abyssi
L-ornithine + acetate
-
-
-
?
N2-acety-L-ornithine + H2O
step in arginine biosynthesis
171963
Escherichia coli
L-ornithine + acetate
-
-
-
?
N2-acety-L-ornithine + H2O
step in arginine biosynthesis
171963
Moritella profunda
L-ornithine + acetate
-
-
-
?
N2-acety-L-ornithine + H2O
step in arginine biosynthesis
171963
Escherichia coli 160-37
L-ornithine + acetate
-
-
-
?
N2-acety-L-ornithine + H2O
step in arginine biosynthesis
171963
Escherichia coli ATCC 9637
L-ornithine + acetate
-
-
-
?
Nalpha-acetyl-L-ornithine + H2O
-
171963
Escherichia coli
L-ornithine + acetate
-
-
-
?
Nalpha-acetyl-L-ornithine + H2O
-
171963
Moritella profunda
L-ornithine + acetate
-
-
-
?
Nalpha-acetyl-L-ornithine + H2O
-
171963
Escherichia coli 160-37
L-ornithine + acetate
-
-
-
?
Nalpha-acetyl-L-ornithine + H2O
-
171963
Escherichia coli ATCC 9637
L-ornithine + acetate
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
AOase
-
Escherichia coli
AOase
-
Moritella profunda
enterobacterial AOase
-
Escherichia coli
enterobacterial AOase
-
Moritella profunda
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
transformed with plasmid pC-28 harboring argE from strain 2693
Escherichia coli
40
-
-
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
Escherichia coli transformed with pTrc99A + pC-28 containing argE, lambdaZAP library from 2674
Escherichia coli
Escherichia coli transformed with pTrc99A + pC-28 containing argE, lambdaZAP library from 2674
Moritella profunda
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
N2-acety-L-ornithine + H2O
Moritella abyssi
-
L-ornithine + acetate
-
-
?
N2-acety-L-ornithine + H2O
Escherichia coli
step in arginine biosynthesis
L-ornithine + acetate
-
-
?
N2-acety-L-ornithine + H2O
Moritella profunda
step in arginine biosynthesis
L-ornithine + acetate
-
-
?
N2-acety-L-ornithine + H2O
Escherichia coli 160-37
step in arginine biosynthesis
L-ornithine + acetate
-
-
?
N2-acety-L-ornithine + H2O
Escherichia coli ATCC 9637
step in arginine biosynthesis
L-ornithine + acetate
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
N2-acety-L-ornithine + H2O
-
171963
Moritella abyssi
L-ornithine + acetate
-
-
-
?
N2-acety-L-ornithine + H2O
step in arginine biosynthesis
171963
Escherichia coli
L-ornithine + acetate
-
-
-
?
N2-acety-L-ornithine + H2O
step in arginine biosynthesis
171963
Moritella profunda
L-ornithine + acetate
-
-
-
?
N2-acety-L-ornithine + H2O
step in arginine biosynthesis
171963
Escherichia coli 160-37
L-ornithine + acetate
-
-
-
?
N2-acety-L-ornithine + H2O
step in arginine biosynthesis
171963
Escherichia coli ATCC 9637
L-ornithine + acetate
-
-
-
?
Nalpha-acetyl-L-ornithine + H2O
-
171963
Escherichia coli
L-ornithine + acetate
-
-
-
?
Nalpha-acetyl-L-ornithine + H2O
-
171963
Moritella profunda
L-ornithine + acetate
-
-
-
?
Nalpha-acetyl-L-ornithine + H2O
-
171963
Escherichia coli 160-37
L-ornithine + acetate
-
-
-
?
Nalpha-acetyl-L-ornithine + H2O
-
171963
Escherichia coli ATCC 9637
L-ornithine + acetate
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
transformed with plasmid pC-28 harboring argE from strain 2693
Escherichia coli
40
-
-
Escherichia coli
Other publictions for EC 3.5.1.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
755033
Molesini
The Arabidopsis N-acetylornit ...
Arabidopsis thaliana
Plant Cell Physiol.
58
130-144
2017
-
-
-
-
-
-
-
1
-
-
-
1
-
10
-
-
-
-
-
-
-
-
2
-
3
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
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1
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1
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
1
1
-
1
1
734884
Molesini
Involvement of the putative N- ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Plant Cell Physiol.
56
1084-1096
2015
-
-
1
-
1
-
-
-
-
-
-
2
-
12
-
-
-
-
-
9
-
-
2
-
4
-
-
-
-
-
-
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-
-
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-
-
1
-
-
1
-
-
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-
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2
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-
9
-
-
2
-
-
-
-
-
-
-
-
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-
2
2
-
-
-
754657
Hernandez
Genetic and biochemical chara ...
Sinorhizobium meliloti
Microbiology
161
1671-1682
2015
-
-
1
-
-
-
1
1
-
2
-
1
-
12
-
-
1
-
-
-
-
-
1
-
3
1
-
-
-
1
-
-
-
-
-
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1
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-
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-
1
-
1
-
2
-
1
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-
1
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-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
733082
Hlavacek
Mono-N-acyl-2,6-diaminopimelic ...
Escherichia coli
Anal. Biochem.
467
4-13
2014
-
-
-
-
-
-
6
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
4
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
735279
McGregor
Identification of a histidine ...
Escherichia coli
SpringerPlus
2
482
2013
-
-
1
-
4
-
-
-
-
2
-
-
-
5
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
1
-
-
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-
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1
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4
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2
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1
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-
-
-
-
-
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-
1
-
-
-
-
1
1
-
-
-
720166
Tao
Structural characterization of ...
Escherichia coli
J. Inorg. Biochem.
111
157-163
2012
-
-
-
-
-
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-
2
-
-
1
-
-
6
-
-
1
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-
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1
1
1
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2
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2
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1
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1
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-
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
710829
Hlavacek
Inhibitors of N(alpha)-acetyl- ...
Bacillus subtilis, Bacillus subtilis 168
Amino Acids
38
1155-1164
2010
-
-
-
-
-
-
19
-
-
-
-
-
-
98
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
18
19
-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687815
McGregor
Characterization of the cataly ...
Escherichia coli
J. Biol. Inorg. Chem.
12
603-613
2007
-
-
-
-
-
-
1
2
-
1
1
-
-
8
-
-
1
-
-
-
-
-
1
-
2
-
-
1
3
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
2
-
1
1
-
-
-
-
1
-
-
-
-
1
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
669046
McGregor
argE-encoded N-acetyl-L-ornith ...
Escherichia coli, Escherichia coli BL21 (DE3)
J. Am. Chem. Soc.
127
14100-14107
2005
-
1
-
-
-
-
-
2
-
3
3
-
-
15
-
-
1
-
-
-
1
-
2
2
2
-
1
1
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
3
3
-
-
-
-
1
-
-
1
-
2
2
-
1
1
2
-
-
-
-
-
-
-
-
-
-
171963
Xu
Evolution of arginine biosynth ...
Escherichia coli, Moritella profunda, Moritella abyssi, Escherichia coli 160-37, Escherichia coli ATCC 9637
J. Bacteriol.
182
1609-1615
2000
-
-
2
-
-
-
-
-
-
-
-
5
-
32
-
-
-
-
-
-
-
-
9
-
4
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
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-
-
5
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-
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-
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-
9
-
2
-
-
-
-
-
-
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-
-
-
-
-
-
171964
Javid-Majd
Mechanistic analysis of the ar ...
Escherichia coli, Escherichia coli 160-37, Escherichia coli ATCC 9637
Biochemistry
39
1285-1293
2000
1
-
1
-
-
-
5
15
-
4
3
1
-
16
-
-
1
-
-
-
-
-
23
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
1
-
-
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-
-
5
-
15
-
4
3
1
-
-
-
1
-
-
-
-
23
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
171961
Boyen
Acetylornithine deacetylase, s ...
Escherichia coli
Gene
116
1-6
1992
-
-
1
-
-
-
-
-
-
2
3
1
-
8
-
-
1
-
-
-
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-
3
1
1
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1
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2
3
1
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1
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3
1
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-
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-
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-
-
171962
Meinnel
Structural and biochemical cha ...
Escherichia coli, Saccharomyces sp., Escherichia coli 160-37, Escherichia coli ATCC 9637
J. Bacteriol.
174
2323-2331
1992
-
-
1
-
-
-
-
1
-
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5
4
-
21
-
-
1
-
-
-
1
-
15
1
1
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-
-
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-
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1
-
-
-
-
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1
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-
5
4
-
-
-
1
-
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1
-
15
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725629
Van de Casteele
-
Pathways of arginine biosynthe ...
Saccharolobus solfataricus, Saccharolobus solfataricus P1
J. Gen. Microbiol.
136
1177-1183
1990
-
-
-
-
-
-
-
-
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10
-
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2
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2
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1
1
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1
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2
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2
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1
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1
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1
1
1
1
-
-
171960
Vogel
Acetylornithinase of Escherich ...
Aerobacter sp., Escherichia coli, Erwinia sp., Klebsiella sp., Proteus sp., Salmonella sp., Serratia sp., Shigella sp., Escherichia coli B / ATCC 11303, Escherichia coli 160-37, Escherichia coli ATCC 9637
J. Biol. Chem.
218
97-106
1956
1
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-
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5
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2
-
11
-
199
-
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1
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1
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33
-
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1
1
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1
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5
-
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2
-
11
-
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1
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1
-
33
-
-
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-
1
1
-
-
-
-
-
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